Agarose gel drying out during experiment - The Slime Mould Collective2024-03-29T07:01:57Zhttp://slimoco.ning.com/forum/topics/agarose-gel-drying-out-during-experiment?commentId=3917201%3AComment%3A157562&xg_source=activity&feed=yes&xn_auth=noI replied via email but so ot…tag:slimoco.ning.com,2022-01-11:3917201:Comment:1575622022-01-11T09:48:55.071Zianhttp://slimoco.ning.com/profile/ian
<p>I replied via email but so others can see. Agar dries out fast, to deal with it you can enclose the whole setup ( camera included ) in a humidity chamber - which can just be a cardboard frame lined with plastic bag and a pad of wet cloth/paper under the samples.</p>
<p>You can also leave the lid on your dishes, as long as you don't get too much temperature fluctuation a couple of 1mm holes drilled around the edge of the lid will keep in moisture but allow enough air exchange to stop…</p>
<p>I replied via email but so others can see. Agar dries out fast, to deal with it you can enclose the whole setup ( camera included ) in a humidity chamber - which can just be a cardboard frame lined with plastic bag and a pad of wet cloth/paper under the samples.</p>
<p>You can also leave the lid on your dishes, as long as you don't get too much temperature fluctuation a couple of 1mm holes drilled around the edge of the lid will keep in moisture but allow enough air exchange to stop fogging. </p>
<p>I made my agar thicker - 1cm and put pieces of it in a tray with 0.5cm water ( which you will have to top up daily ), a square of paper or cellophane on the top of each piece might give you a cleaner background if you're filming for analysis.</p>