And so it begins.. - The Slime Mould Collective2024-03-28T18:19:06Zhttp://slimoco.ning.com/forum/topics/and-so-it-begins?feed=yes&xn_auth=noIt does seem to take several…tag:slimoco.ning.com,2019-11-25:3917201:Comment:477262019-11-25T10:48:58.915Zianhttp://slimoco.ning.com/profile/ian
<p>It does seem to take several weeks for them to really get going. This week I tried streaking out spores directly onto agar - 1% oat agar and 1% boiled yeast, neither sterile.</p>
<p>First colony is now about 5cm across and is looking like it's about to hit exponential phase.</p>
<p>Nine new colonies on the top right dish ( the agar slices in one dish )!</p>
<p>If all of these come up positive I'll have an accommodation problem!</p>
<p>It does seem to take several weeks for them to really get going. This week I tried streaking out spores directly onto agar - 1% oat agar and 1% boiled yeast, neither sterile.</p>
<p>First colony is now about 5cm across and is looking like it's about to hit exponential phase.</p>
<p>Nine new colonies on the top right dish ( the agar slices in one dish )!</p>
<p>If all of these come up positive I'll have an accommodation problem!</p> Hey Ian,
I've been watching…tag:slimoco.ning.com,2019-11-15:3917201:Comment:473162019-11-15T16:43:10.695ZMark Skellyhttp://slimoco.ning.com/profile/MarkSkelly
<p>Hey Ian,</p>
<p></p>
<p>I've been watching this and it looks great. I'm doing the opposite now. Trying to coax some fruiting bodies into producing sporangium. Nothing as exacting as your processes, but if I get the light levels right (finding the right spot in my apartment) I might get lucky.</p>
<p>I've noticed as well that the cloudy young plasmodium stage is very slow, but I'm alway amazed that when it goes full on diploid the growth is practically exponential. I keep my petri dishes in…</p>
<p>Hey Ian,</p>
<p></p>
<p>I've been watching this and it looks great. I'm doing the opposite now. Trying to coax some fruiting bodies into producing sporangium. Nothing as exacting as your processes, but if I get the light levels right (finding the right spot in my apartment) I might get lucky.</p>
<p>I've noticed as well that the cloudy young plasmodium stage is very slow, but I'm alway amazed that when it goes full on diploid the growth is practically exponential. I keep my petri dishes in sealed plastic bags as Physarum is quite the escape artist.</p> Further reading - way back to…tag:slimoco.ning.com,2019-11-13:3917201:Comment:476042019-11-13T13:08:48.706Zianhttp://slimoco.ning.com/profile/ian
<p>Further reading - way back to 1968 now, temperature appears to be critical no other sod's mentioned that in a paper. Pausing to build an incubator.... In the meantime, raising more spores...</p>
<p>Further reading - way back to 1968 now, temperature appears to be critical no other sod's mentioned that in a paper. Pausing to build an incubator.... In the meantime, raising more spores...</p> Our youngster grows slowly bu…tag:slimoco.ning.com,2019-11-11:3917201:Comment:470992019-11-11T06:38:04.923Zianhttp://slimoco.ning.com/profile/ian
<p>Our youngster grows slowly but is getting there - the only colony so far. I've been reading papers from the 60s and 70s in which amino benzoic acids were used as supplements to encourage plasmodia. I'm now trying mushroom extract - should be able to get this to a reliable 7 day turn around, in the mean time - raising spores </p>
<p>Our youngster grows slowly but is getting there - the only colony so far. I've been reading papers from the 60s and 70s in which amino benzoic acids were used as supplements to encourage plasmodia. I'm now trying mushroom extract - should be able to get this to a reliable 7 day turn around, in the mean time - raising spores </p> Well I wasn't expecting anyth…tag:slimoco.ning.com,2019-11-02:3917201:Comment:464342019-11-02T13:33:05.552Zianhttp://slimoco.ning.com/profile/ian
<p>Well I wasn't expecting anything at just six days but...</p>
<p><a href="https://storage.ning.com/topology/rest/1.0/file/get/3693651812?profile=original" rel="noopener" target="_blank"><img class="align-center" src="https://storage.ning.com/topology/rest/1.0/file/get/3693651812?profile=RESIZE_710x" width="400"></img></a> Sorry about image quality - phone held up to 'scope eyepiece, I need to find drivers for the USB microscope.</p>
<p>This is from A2 2.5cm whatman paper disc.</p>
<p></p>
<p>Next step is to get it off there and into its own disk for bulking up…</p>
<p></p>
<p>Well I wasn't expecting anything at just six days but...</p>
<p><a href="https://storage.ning.com/topology/rest/1.0/file/get/3693651812?profile=original" target="_blank" rel="noopener"><img src="https://storage.ning.com/topology/rest/1.0/file/get/3693651812?profile=RESIZE_710x" class="align-center" width="400"/></a>Sorry about image quality - phone held up to 'scope eyepiece, I need to find drivers for the USB microscope.</p>
<p>This is from A2 2.5cm whatman paper disc.</p>
<p></p>
<p>Next step is to get it off there and into its own disk for bulking up</p>
<p><a href="https://storage.ning.com/topology/rest/1.0/file/get/3693654808?profile=original" target="_blank" rel="noopener"><img src="https://storage.ning.com/topology/rest/1.0/file/get/3693654808?profile=RESIZE_710x" class="align-center" width="320"/></a>That oat should be ready to move tomorrow then it's bulk up, make some scletrotia and then see what this thing can do.</p>
<p></p>
<p><a href="https://storage.ning.com/topology/rest/1.0/file/get/3693656367?profile=original" target="_blank" rel="noopener"><img src="https://storage.ning.com/topology/rest/1.0/file/get/3693656367?profile=RESIZE_710x" class="align-center" width="340"/></a>This looks like it might be another one - the very young plasmodia lack pigment. This one ( if it is a slime ) is from JM which is pale to start with.</p>
<p></p>