cryopreservation of physarum polycephalum - The Slime Mould Collective2024-03-29T11:36:00Zhttp://slimoco.ning.com/forum/topics/cryopreservation-of-physarum-polycephalum?commentId=3917201%3AComment%3A25105&feed=yes&xn_auth=noLeft it in the draw!! You hav…tag:slimoco.ning.com,2018-01-15:3917201:Comment:383282018-01-15T10:47:35.194ZDr Jason bellhttp://slimoco.ning.com/profile/jasonbell
<p>Left it in the draw!! You have just disclosed, one of the most sacred secrets of the science lab :D</p>
<p>Left it in the draw!! You have just disclosed, one of the most sacred secrets of the science lab :D</p> Hi AllWelcome to September. I…tag:slimoco.ning.com,2016-09-22:3917201:Comment:315082016-09-22T01:52:53.807ZWarren Tanhttp://slimoco.ning.com/profile/Warren
<p>Hi All<br></br><br></br>Welcome to September. I manged to revive the dried sclerotium kept at -80°C since July last year. :)</p>
<p>Placed the sclerotium on 1% bacto agar, and on the next day it is spreading out hunting for fresh oats already.</p>
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<p><a href="http://storage.ning.com/topology/rest/1.0/file/get/308443159?profile=original" target="_self"><img class="align-center" src="http://storage.ning.com/topology/rest/1.0/file/get/308443159?profile=RESIZE_1024x1024" width="721"></img></a> <br></br><br></br>And... freezing at -80°C saved my culture. Probably because I am in warm and humid Singapore, all my sclerotium…</p>
<p>Hi All<br><br>Welcome to September. I manged to revive the dried sclerotium kept at -80°C since July last year. :)</p>
<p>Placed the sclerotium on 1% bacto agar, and on the next day it is spreading out hunting for fresh oats already.</p>
<p></p>
<p><a target="_self" href="http://storage.ning.com/topology/rest/1.0/file/get/308443159?profile=original"><img class="align-center" src="http://storage.ning.com/topology/rest/1.0/file/get/308443159?profile=RESIZE_1024x1024" width="721"></a><br><br>And... freezing at -80°C saved my culture. Probably because I am in warm and humid Singapore, all my sclerotium kept at ambient condition didn't revive. Oddly, that is despite my lab being air conditioned at 25°C... maybe because ambient humidity is still too high.<br><br>Warren</p> Make sure you take some with…tag:slimoco.ning.com,2015-07-13:3917201:Comment:251122015-07-13T09:12:50.112Zianhttp://slimoco.ning.com/profile/ian
<p>Make sure you take some with you!</p>
<p>As for storage, since half of cryo storage is avoiding ice crystals I'd suggest freezing sclerotia as Warren has tries is the way to go - it's already low enough in water to put up with a snap freeze. I'd also just dry down a load and leave it 'ambient temperature' - which as we all know is science speak for 'we just left it in the drawer' </p>
<p>You could hedge your bet and store some spores too.</p>
<p>Make sure you take some with you!</p>
<p>As for storage, since half of cryo storage is avoiding ice crystals I'd suggest freezing sclerotia as Warren has tries is the way to go - it's already low enough in water to put up with a snap freeze. I'd also just dry down a load and leave it 'ambient temperature' - which as we all know is science speak for 'we just left it in the drawer' </p>
<p>You could hedge your bet and store some spores too.</p> Thanks two of you!I just want…tag:slimoco.ning.com,2015-07-12:3917201:Comment:252072015-07-12T06:29:25.755ZChoi Ming Hohttp://slimoco.ning.com/profile/ChoiMingHo
<p>Thanks two of you!<br/>I just want to make a longer term storage, as long as possible, since I will leave my university soon and maybe no one would take up the rearing job.</p>
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<p>Thanks two of you!<br/>I just want to make a longer term storage, as long as possible, since I will leave my university soon and maybe no one would take up the rearing job.</p>
<p></p> Why freeze it all though? the…tag:slimoco.ning.com,2015-07-09:3917201:Comment:252042015-07-09T09:22:41.794Zianhttp://slimoco.ning.com/profile/ian
<p>Why freeze it all though? the sclerotia store fine</p>
<p>Why freeze it all though? the sclerotia store fine</p> Recently i tried freezing the…tag:slimoco.ning.com,2015-07-09:3917201:Comment:251052015-07-09T09:20:45.906ZWarren Tanhttp://slimoco.ning.com/profile/Warren
<p>Recently i tried freezing the dried sclerotium at -80°C. They recovered ok after a week of freezing, will take another aliquot for thaw-recovery test in a few months' time to be sure.<br/><br/>I guess that could be an option. :)</p>
<p>Recently i tried freezing the dried sclerotium at -80°C. They recovered ok after a week of freezing, will take another aliquot for thaw-recovery test in a few months' time to be sure.<br/><br/>I guess that could be an option. :)</p> You'd need to get them smalle…tag:slimoco.ning.com,2015-07-08:3917201:Comment:251022015-07-08T10:28:35.806Zianhttp://slimoco.ning.com/profile/ian
<p>You'd need to get them smaller and into liquid culture to stand a chance - there are details here though not of freezing: <a href="http://www.researchgate.net/publication/251181953_Microplasmodium_Dynamics_of_Physarum_Polycephalum" target="_blank">http://www.researchgate.net/publication/251181953_Microplasmodium_Dynamics_of_Physarum_Polycephalum</a></p>
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<p>Why freeze when the sclerotium is viable for years though? Just dry a plasmodium out on paper and cut it…</p>
<p>You'd need to get them smaller and into liquid culture to stand a chance - there are details here though not of freezing: <a href="http://www.researchgate.net/publication/251181953_Microplasmodium_Dynamics_of_Physarum_Polycephalum" target="_blank">http://www.researchgate.net/publication/251181953_Microplasmodium_Dynamics_of_Physarum_Polycephalum</a></p>
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<p>Why freeze when the sclerotium is viable for years though? Just dry a plasmodium out on paper and cut it up. </p>
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<p></p> I had the same intention of f…tag:slimoco.ning.com,2015-06-26:3917201:Comment:250282015-06-26T09:43:52.600ZWarren Tanhttp://slimoco.ning.com/profile/Warren
I had the same intention of freezing slime mould at -80degrees for long term storage!<br />
<br />
However I had problem trying to get slime mould to grow in liquid (tried both LB broth and oat porridge), and hence it is hard to mix in glycerol.<br />
<br />
Freezing slime mould covered oats submerged in glycerol didn't work.<br />
<br />
Suggestions welcome! :p
I had the same intention of freezing slime mould at -80degrees for long term storage!<br />
<br />
However I had problem trying to get slime mould to grow in liquid (tried both LB broth and oat porridge), and hence it is hard to mix in glycerol.<br />
<br />
Freezing slime mould covered oats submerged in glycerol didn't work.<br />
<br />
Suggestions welcome! :p