New to slime moulds -- How many times can I grow a dish out and inoculate other dish with part of the original mould? Endlessly? - The Slime Mould Collective2024-03-29T05:33:42Zhttp://slimoco.ning.com/forum/topics/new-to-slime-moulds-how-many-times-can-i-grow-a-dish-out-and?commentId=3917201%3AComment%3A157242&feed=yes&xn_auth=noWow!tag:slimoco.ning.com,2022-01-12:3917201:Comment:1575652022-01-12T12:26:27.097ZKatharinehttp://slimoco.ning.com/profile/Katharine
<p>Wow!</p>
<p>Wow!</p> You'd send me some? That'd b…tag:slimoco.ning.com,2021-11-23:3917201:Comment:1569562021-11-23T17:45:38.497Zsuperuserhttp://slimoco.ning.com/profile/HaydenHunley
<p>You'd send me some? That'd be so greatly appreciated. I'm in the US if that matters for if you can or not. I will DM you with some of my info and I'll pay whatever the price and shipping you say is</p>
<p>You'd send me some? That'd be so greatly appreciated. I'm in the US if that matters for if you can or not. I will DM you with some of my info and I'll pay whatever the price and shipping you say is</p> The egg is made up in agar in…tag:slimoco.ning.com,2021-11-23:3917201:Comment:1571362021-11-23T08:33:10.286Zianhttp://slimoco.ning.com/profile/ian
<p>The egg is made up in agar in the literature, I've also mixed it in with oats. </p>
<p>I can send you slime....</p>
<p>The egg is made up in agar in the literature, I've also mixed it in with oats. </p>
<p>I can send you slime....</p> Nice thanks for the answers! …tag:slimoco.ning.com,2021-11-17:3917201:Comment:1570322021-11-17T22:57:28.632Zsuperuserhttp://slimoco.ning.com/profile/HaydenHunley
<p>Nice thanks for the answers! It makes sense that they'd just make a large circle if everything around them is just food! By 10% powdered egg, what's the other 90%? Or is 10% like just how you buy it I've never heard of the stuff.</p>
<p></p>
<p>For the jar -- I was thinking of pressure cooking to sterilize the oats, but also having holes on the lid with micropore tape to let it breathe! (something very common in mycology)</p>
<p></p>
<p>Now to just get my hands on one of them :^)</p>
<p>Nice thanks for the answers! It makes sense that they'd just make a large circle if everything around them is just food! By 10% powdered egg, what's the other 90%? Or is 10% like just how you buy it I've never heard of the stuff.</p>
<p></p>
<p>For the jar -- I was thinking of pressure cooking to sterilize the oats, but also having holes on the lid with micropore tape to let it breathe! (something very common in mycology)</p>
<p></p>
<p>Now to just get my hands on one of them :^)</p> I've just recently found that…tag:slimoco.ning.com,2021-11-17:3917201:Comment:1569452021-11-17T16:02:27.590ZAndyGadgethttp://slimoco.ning.com/profile/AndyStansfield
<p>I've just recently found that my Physarum polycephalum prefers muesli oats to the porridge oats I was feeding it. It would also enclose a piece of crystallised ginger (also from the muesli) but suprisingly, not interested in a bit of raisin. </p>
<p>I've just recently found that my Physarum polycephalum prefers muesli oats to the porridge oats I was feeding it. It would also enclose a piece of crystallised ginger (also from the muesli) but suprisingly, not interested in a bit of raisin. </p> Hi
Yeah - in principle you s…tag:slimoco.ning.com,2021-11-17:3917201:Comment:1572422021-11-17T14:04:06.614Zianhttp://slimoco.ning.com/profile/ian
<p>Hi </p>
<p>Yeah - in principle you should be able to keep one going indefinitely. The Dusstour lab do theirs by half filling a petri dish with a semicircle of oats, adding slime to that then filling the other side and scooping out the old. </p>
<p>In practise they do tend to go down with something stinky in the end - you could sterilise all your food but oats are rich and contaminant prone.</p>
<p></p>
<p>As for agar - I'd keep them on paper if I were you for day to day growing - it's…</p>
<p>Hi </p>
<p>Yeah - in principle you should be able to keep one going indefinitely. The Dusstour lab do theirs by half filling a petri dish with a semicircle of oats, adding slime to that then filling the other side and scooping out the old. </p>
<p>In practise they do tend to go down with something stinky in the end - you could sterilise all your food but oats are rich and contaminant prone.</p>
<p></p>
<p>As for agar - I'd keep them on paper if I were you for day to day growing - it's cheaper. The reason to use water agar is slimes don't form patterns without a gradient to follow ( usually a food gradient ), if you grow them on nute agar they just spread out into a uniform yellow sheet and sit there. Btw if you want a really strong attractant, 10% powdered egg gets them moving.</p>
<p></p>
<p>Filling a jar - they'd grow, I wonder how far down though as they're aerobic. It's worth a try, you'd need to sterilise the oats then hydrate with sterile water or you'd just end up with a solid block of porridge. </p>