Prefered substrate - The Slime Mould Collective2024-03-28T11:37:07Zhttp://slimoco.ning.com/forum/topics/prefered-substrate?feed=yes&xn_auth=noNutrient agar isn't a great o…tag:slimoco.ning.com,2021-08-24:3917201:Comment:1553202021-08-24T09:29:56.334Zianhttp://slimoco.ning.com/profile/ian
<p>Nutrient agar isn't a great option - you need it autoclaved to be sterile and it's prone to growing contaminants. Slime will grow on it but it only forms structures when there's a gradient of food available so on nutrient agar it just makes a sheet.</p>
<p>I'm using cartridge paper ( the stuff that's too think for paper l, too thin for card) and brown kraft paper - for both I either spray or pour on water and pour off what doesn't soak in.</p>
<p>I did find coffee filters aren't great - the…</p>
<p>Nutrient agar isn't a great option - you need it autoclaved to be sterile and it's prone to growing contaminants. Slime will grow on it but it only forms structures when there's a gradient of food available so on nutrient agar it just makes a sheet.</p>
<p>I'm using cartridge paper ( the stuff that's too think for paper l, too thin for card) and brown kraft paper - for both I either spray or pour on water and pour off what doesn't soak in.</p>
<p>I did find coffee filters aren't great - the pores are large enough for the slime to get through. </p> What a terrific topic, thanks…tag:slimoco.ning.com,2021-08-19:3917201:Comment:1549632021-08-19T13:33:40.984ZFred Bartletthttp://slimoco.ning.com/profile/Bartlett
<p>What a terrific topic, thanks for starting this, Nuno. </p>
<p>So far I've used agar, somehow I don't remember filter paper working that well. Maybe I kept it too wet or too dry? How does one handle moisture levels with fitler paper? The agar agar already has water in it, so that really isn't an issue.</p>
<p></p>
<p>Has anone tried making a media with food already incorporated into it, such as powdered oates in the agar medium? I'll give Ian's idea of just using oats themselves as a growing…</p>
<p>What a terrific topic, thanks for starting this, Nuno. </p>
<p>So far I've used agar, somehow I don't remember filter paper working that well. Maybe I kept it too wet or too dry? How does one handle moisture levels with fitler paper? The agar agar already has water in it, so that really isn't an issue.</p>
<p></p>
<p>Has anone tried making a media with food already incorporated into it, such as powdered oates in the agar medium? I'll give Ian's idea of just using oats themselves as a growing medium a try too...!</p> Hi
Paper's definitely easier.…tag:slimoco.ning.com,2021-08-17:3917201:Comment:1553062021-08-17T06:26:26.970Zianhttp://slimoco.ning.com/profile/ian
<p>Hi</p>
<p>Paper's definitely easier. Another method I've seen in labs is to just fill a petri with oats and innoculate, when it's filled the dish half is dug out and the gap refilled with fresh oats </p>
<p>Hi</p>
<p>Paper's definitely easier. Another method I've seen in labs is to just fill a petri with oats and innoculate, when it's filled the dish half is dug out and the gap refilled with fresh oats </p>