Problems with raising Physarum Polycephalum from sclerotia - The Slime Mould Collective2024-03-29T14:02:49Zhttp://slimoco.ning.com/forum/topics/problems-with-raising-physarum-polycephalum-from-sclerotia?commentId=3917201%3AComment%3A97934&feed=yes&xn_auth=noFYI I'm having a similar prob…tag:slimoco.ning.com,2021-01-22:3917201:Comment:1249452021-01-22T04:32:05.277ZMPhttp://slimoco.ning.com/profile/MP
<p>FYI I'm having a similar problem as well, for the past month or so, with Mazie + Daywalker.<br/><br/>Age: not an issue, I received these from Ian in August! worked fine then~<br/>Temp: year-round temps here are 25-30'C.<br/>Humidity: it's more humid now, actually</p>
<p></p>
<p>I'll try the "go for broke" method now.</p>
<p>FYI I'm having a similar problem as well, for the past month or so, with Mazie + Daywalker.<br/><br/>Age: not an issue, I received these from Ian in August! worked fine then~<br/>Temp: year-round temps here are 25-30'C.<br/>Humidity: it's more humid now, actually</p>
<p></p>
<p>I'll try the "go for broke" method now.</p> Update:One Daywalker managed…tag:slimoco.ning.com,2020-12-04:3917201:Comment:984512020-12-04T13:16:48.495ZTymon Zacharyhttp://slimoco.ning.com/profile/TymonZachary
<p>Update:<br/>One Daywalker managed to develop and it seems pretty normal, the second I sprinkled with crushed oats and I think it helped. I'm going to observe them. My other trials proved unsuccessful. I'm going to have a try of this last-chance method today.</p>
<p>Thank you very much for your help both substantive and material.</p>
<p>Update:<br/>One Daywalker managed to develop and it seems pretty normal, the second I sprinkled with crushed oats and I think it helped. I'm going to observe them. My other trials proved unsuccessful. I'm going to have a try of this last-chance method today.</p>
<p>Thank you very much for your help both substantive and material.</p> Hi
Your best bet is go for b…tag:slimoco.ning.com,2020-12-04:3917201:Comment:984172020-12-04T06:38:56.536Zianhttp://slimoco.ning.com/profile/ian
<p>Hi </p>
<p>Your best bet is go for broke - take the whole remaining piece, hydrate it and add some powdered oats straight on top - just a pinch. Keep them at 20c min, when you see growth, feed slowly.</p>
<p>It's possible you just got a bad batch of sclerotia, but since both strains are bad, I wonder if they got overheated or something in the post.</p>
<p>I'll post you more on Saturday - they do come with a guarantee! </p>
<p>Hi </p>
<p>Your best bet is go for broke - take the whole remaining piece, hydrate it and add some powdered oats straight on top - just a pinch. Keep them at 20c min, when you see growth, feed slowly.</p>
<p>It's possible you just got a bad batch of sclerotia, but since both strains are bad, I wonder if they got overheated or something in the post.</p>
<p>I'll post you more on Saturday - they do come with a guarantee! </p> Thank you very much, your ans…tag:slimoco.ning.com,2020-12-03:3917201:Comment:978732020-12-03T15:20:31.887ZTymon Zacharyhttp://slimoco.ning.com/profile/TymonZachary
Thank you very much, your answer was the thing I hoped for for I know you're an expert according slime moulds (or molds, it confuses me which one is correct). The strains are both Daywalker and Mazie which I recieved from you in the summer. I also expected this answer because slimes from my sclerotia seemed all right. As you said, the pigment leaked after rehydrating. But I don't quite get what am I to do. Shall I sprinkle my not vigorous slimes with powdered oats? Am I to put freshly…
Thank you very much, your answer was the thing I hoped for for I know you're an expert according slime moulds (or molds, it confuses me which one is correct). The strains are both Daywalker and Mazie which I recieved from you in the summer. I also expected this answer because slimes from my sclerotia seemed all right. As you said, the pigment leaked after rehydrating. But I don't quite get what am I to do. Shall I sprinkle my not vigorous slimes with powdered oats? Am I to put freshly rehydrated sclerotia into a fridge? Can these sclerotia be mke useful again in any way? Hi
What's the strain and how…tag:slimoco.ning.com,2020-12-03:3917201:Comment:979482020-12-03T13:55:37.184Zianhttp://slimoco.ning.com/profile/ian
<p>Hi</p>
<p>What's the strain and how old are the sclerotia? If they're leaking a load of pigment soon after rehydrating, it's the sclerotia that's the problem - comes with an earthy smell. Cold down to 4c won't kill them - I store mine in the fridge occasionally. An hour to two ours is about right for a newly awoken slime to cover an oat flake though. Finely powdered oats can help revive a weak one. </p>
<p>Hi</p>
<p>What's the strain and how old are the sclerotia? If they're leaking a load of pigment soon after rehydrating, it's the sclerotia that's the problem - comes with an earthy smell. Cold down to 4c won't kill them - I store mine in the fridge occasionally. An hour to two ours is about right for a newly awoken slime to cover an oat flake though. Finely powdered oats can help revive a weak one. </p> It's true that humidity drops…tag:slimoco.ning.com,2020-12-03:3917201:Comment:980312020-12-03T13:45:07.864ZTymon Zacharyhttp://slimoco.ning.com/profile/TymonZachary
It's true that humidity drops when temperature falls down, but the slimes are keeped in the petri dishes so, I guess, it shouldn't affect them. Nevertheless, I'm grateful for your response.
It's true that humidity drops when temperature falls down, but the slimes are keeped in the petri dishes so, I guess, it shouldn't affect them. Nevertheless, I'm grateful for your response. Just a guess, but could it be…tag:slimoco.ning.com,2020-12-03:3917201:Comment:979342020-12-03T10:14:56.453ZRyChuhttp://slimoco.ning.com/profile/Raichu
<p>Just a guess, but could it be the humidity? Seems like temperature is accounted for, not sure if humidity is dropping too.</p>
<p>Just a guess, but could it be the humidity? Seems like temperature is accounted for, not sure if humidity is dropping too.</p>