RADIOACTIVE SLIME! - The Slime Mould Collective2024-03-28T22:57:01Zhttp://slimoco.ning.com/forum/topics/radioactive-slime?commentId=3917201%3AComment%3A48986&feed=yes&xn_auth=noYou dare me? Well I guess I h…tag:slimoco.ning.com,2020-04-06:3917201:Comment:492652020-04-06T06:46:20.126ZEvan Brownhttp://slimoco.ning.com/profile/EvanBrown
<p>You dare me? Well I guess I have no choice then.</p>
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<p>You dare me? Well I guess I have no choice then.</p>
<p></p> Do it I dare you. Then post a…tag:slimoco.ning.com,2020-04-05:3917201:Comment:490522020-04-05T23:59:17.582ZRyChuhttp://slimoco.ning.com/profile/Raichu
<p>Do it I dare you. Then post a video.</p>
<p>Do it I dare you. Then post a video.</p> Lol, more of a "WEEEEEEEEEEEE…tag:slimoco.ning.com,2020-04-05:3917201:Comment:491642020-04-05T01:03:47.977ZEvan Brownhttp://slimoco.ning.com/profile/EvanBrown
<p>Lol, more of a "WEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE, CLICK!!" sound. But I am considering adding a pew pew sound effect just for fun.</p>
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<p>Lol, more of a "WEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE, CLICK!!" sound. But I am considering adding a pew pew sound effect just for fun.</p>
<p></p> Pew pew! [Insert other laser…tag:slimoco.ning.com,2020-04-04:3917201:Comment:491622020-04-04T16:24:59.349ZRyChuhttp://slimoco.ning.com/profile/Raichu
<p>Pew pew! [Insert other laser sounds]</p>
<p>Pew pew! [Insert other laser sounds]</p> I just ordered some strontium…tag:slimoco.ning.com,2020-04-04:3917201:Comment:491602020-04-04T11:43:34.494ZEvan Brownhttp://slimoco.ning.com/profile/EvanBrown
<p>I just ordered some strontium aluminate to try out, I intend to mix it into some oat flour with a bit of Valerian root to see if I can get them to eat that. I think I have some eosin laying around somewhere. I just noticed today that the advancing edge of the plasmodium is showing a much stronger fluorescence than the middle portions. It's really fascinating to watch how it changes.</p>
<p>I wasn't aware of the electroporation, thanks for that info. And it's good to know I don't have to…</p>
<p>I just ordered some strontium aluminate to try out, I intend to mix it into some oat flour with a bit of Valerian root to see if I can get them to eat that. I think I have some eosin laying around somewhere. I just noticed today that the advancing edge of the plasmodium is showing a much stronger fluorescence than the middle portions. It's really fascinating to watch how it changes.</p>
<p>I wasn't aware of the electroporation, thanks for that info. And it's good to know I don't have to shell out the money for a full CRISPR kit. Now I just need to look into getting some GFP plasmid, which I've been wanting to play around with anyway.</p>
<p>That's a shame that you're separated from your lab, but at least you have a class 4 laser. I'm expecting some chromium oxide in the post tomorrow to attempt to make my own synthetic rubies to build a laser out of.</p> I couldn't persuade them to e…tag:slimoco.ning.com,2020-04-04:3917201:Comment:489922020-04-04T09:36:36.039Zianhttp://slimoco.ning.com/profile/ian
<p>I couldn't persuade them to eat strontium aluminate! If you fed another culture rhodamine or eosin and had a couple of cameras you'd be able to follow the mixing ( with two cams you could use R & G filters to separate signals ).</p>
<p>Bioluminesence would be hard - we do use luminescent constructs of cells and plants but in many cases luciferin has to be added and the signal isn't naked eye visible . If you put the full pathway in for luciferin/ase it's a major drain on ATP for the…</p>
<p>I couldn't persuade them to eat strontium aluminate! If you fed another culture rhodamine or eosin and had a couple of cameras you'd be able to follow the mixing ( with two cams you could use R & G filters to separate signals ).</p>
<p>Bioluminesence would be hard - we do use luminescent constructs of cells and plants but in many cases luciferin has to be added and the signal isn't naked eye visible . If you put the full pathway in for luciferin/ase it's a major drain on ATP for the organism.</p>
<p>There are GFP physarum constructs around ( I'd love to get my fingers on one ) - you can neatly tag the GFP to different parts of the cell. Damn I miss the lab - it's been 4 weeks since I shot anything with a class 4 laser.</p>
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<p>You wouldn't need Crispr by the way - physarum you can just bang a plasmid in there, give it an electric shock ( electroporation ) and away you go! I'd put money on just straight injecting it working - it's not a permanent transformation but it's good for hours-days. It might be worth buying a GFP bug kit and just try the plasmid on it.</p>
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<p></p> WOW! Glow-in-the-dark slime w…tag:slimoco.ning.com,2020-04-02:3917201:Comment:489862020-04-02T02:24:44.361ZRyChuhttp://slimoco.ning.com/profile/Raichu
<p>WOW! Glow-in-the-dark slime would be great for display! No light needed.</p>
<p>WOW! Glow-in-the-dark slime would be great for display! No light needed.</p> Oats laced with fluorescein.…tag:slimoco.ning.com,2020-04-02:3917201:Comment:490432020-04-02T02:21:41.357ZEvan Brownhttp://slimoco.ning.com/profile/EvanBrown
<p><span class="module__title__link">Oats laced with fluorescein. I want to try it with strontium aluminate based glow powder so hopefully it will glow in the dark. I have another method I want to eventually look into using CRISPR to insert a bioluminescent gene. No idea how either of those will work out yet though.<br/></span></p>
<p><span class="module__title__link">Oats laced with fluorescein. I want to try it with strontium aluminate based glow powder so hopefully it will glow in the dark. I have another method I want to eventually look into using CRISPR to insert a bioluminescent gene. No idea how either of those will work out yet though.<br/></span></p> Wow! Fascinating! How?tag:slimoco.ning.com,2020-04-02:3917201:Comment:491542020-04-02T02:15:59.986ZRyChuhttp://slimoco.ning.com/profile/Raichu
<p>Wow! Fascinating! How?</p>
<p>Wow! Fascinating! How?</p>