The Slime Mould Collective

An international network of/for intelligent organisms

ian
  • Birmingham
  • United Kingdom
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Ian's Friends

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ian's Discussions

So how do we use slime for evil instead of good?

Started this discussion. Last reply by Dr Jason bell Feb 11. 1 Reply

We're all aware of how great slime moulds are and how they inspire artists, scientists and nutters to perform great deeds.But we're neglecting the darker side of life, how might an evil genius make…Continue

What the???? Interloper?

Started this discussion. Last reply by ian Apr 21, 2017. 1 Reply

I was watching The…Continue

Making a habitat

Started Mar 14, 2017 0 Replies

Some…Continue

Slimy porridge squares - a tidier way to give out physarum

Started this discussion. Last reply by ian Mar 24, 2017. 3 Replies

This might be handy for those of us that tour with slime. I've never liked agar for giving out cultures - it's hard to get a decent density of physarum to make forty odd portions so I grow on paper…Continue

 

ian's Page

Latest Activity

ian replied to Dr Jason bell's discussion Attempt at liquid culture
"Hi This image is 4 days growths to fill a standard petri, that's around 20C. I don't control temp so it varies wildly but even in the summer when RT is around 28C they cope fine. In the lab I do shove them in the server room if I think…"
Feb 12
ian replied to Dr Jason bell's discussion Slime Meets SLIME
"Water beads work really well - I've done time lapses with them,"
Feb 12
Dr Jason bell replied to ian's discussion So how do we use slime for evil instead of good?
"Genetically alter oats, make them produce huge amounts of growth factor, add in some GFP. Once with have six foot tall slime...... Great question, and I have just the class to ask :D"
Feb 11
ian replied to Dr Jason bell's discussion Attempt at liquid culture
"I'm always up for sharing data and I'll keep it confidential.  For choice of bacteria,  what about going free range?  I raise amoebae using whatever grows on rancid oats just by throwing some into water,  it generates…"
Feb 8
ian liked Lauren Loop's photo
Feb 6
ian liked Lauren Loop's photo
Feb 6
John Robinson commented on ian's photo
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Amoeba - triple stain

"Fantastic image :)"
Feb 5
ian posted a photo

Amoeba - triple stain

Physarum amoeba cell under fluorescence. Green - Actin ( stained with phalloidin-488 ), red mitochondria ( Mito-tracker red ), Cyan - nucleus ( stained with hoescht ).Projection from stack of 300 images. Cells in background are various 'wild'…
Feb 5
ian liked John Robinson's photo
Feb 1
ian commented on John Robinson's photo
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Elaeomyxa cerifera 1B centrecrop1000_watermark

"That is gorgeous, what's your setup?"
Feb 1
ian liked John Robinson's photo
Feb 1
John Robinson commented on ian's photo
Jan 31
ian replied to Dr Jason bell's discussion Attempt at liquid culture
"I'm not sure the diploid phase would feed off bacteria in suspension, there's loads in the literature on LC and it's used for bulking up the haploid cells and maintaining them but plasmodial culture is always done in a rich medium.…"
Jan 30
ian commented on Kristy keller's photo
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Physarum polycephalum skin contact

"There are protists that cause disease in humans - acanthamoeba for one.  There's never been any record of a myxomycete causing disease but that doesn't mean it can't happen.  What we can be sure of though is whatever this…"
Jan 30
ian commented on ian's photo
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actin Maximum intensity projection

"Yeh in Amoeboid things actin filaments polymerise ( gel ) at the leading edge extending the cell  and break down at the trailing end ( sol ) contracting it in. In this view we're seeing the stable scaffold of an area that's not…"
Jan 24
Jan-Maarten Luursema commented on ian's photo
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actin Maximum intensity projection

"Awesome! Are these fibers involved in locomotion; do they contract?"
Jan 24

Profile Information

Name
hardboiledparrot
Interest in slime moulds
I'm a professional microscopist and amateur time lapse filmer. I'm looking forward to combining both disciplines with these fascinating organisms
Academic Institution (if applicable)
warwick
Website
http://www.youtube.com/channel/UC5ldTLqKM6QuRQ7zaimMdxg

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Comment Wall (5 comments)

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At 21:03 on January 16, 2018, Kendra Fanconi said…

Oh that would be amazing. I was looking at ordering it from the US. Is there any issue with getting it through customs? 

At 16:05 on October 27, 2015, Marijn Roelvink said…
We received the Physarum today, thanks a lot!
At 12:19 on October 14, 2015, Marijn Roelvink said…
Hi Ian, thank you for wanting to send the Physarum, I was just wondering if you could tell me when they will arrive, it'll help a lot with our planning :)
At 10:37 on February 10, 2015, enrico viola said…
I received my samples today: thanks a lot!
At 8:10 on February 3, 2015, enrico viola said…

Sent you a pm with my address; thanks again!

Ian's Blog

Raising from Spores - Zero Tech Method

Posted on April 11, 2017 at 13:30 0 Comments

I've been looking for a way to rear physarum from spores without resorting to bacterial lawns and needing autoclaves etc.

I've only done a couple of trials but this method seems to work ok, it does require a bit of patience.

I used petri dishes and filter paper but any container and a bit of tissue should work.

The idea is to let the food go off producing bacteria for the amoebal phase to feed off then slowly dry them out so they get persuaded to form…

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Big bang, big disappointment :)

Posted on March 16, 2016 at 17:04 0 Comments

Four weeks of crazy work,  turning the spare room into a culture lab and time lapse studio I made enough culture for two thousand people and I should be there with my friends and colleagues on the stand inspiring the next generation of scientists,  slime fans and eccentrics.  

Unfortunately I'm stuck on a hospital ward with fifty stitches in my neck waiting until I'm allowed home. So it goes I suppose but damn I was looking forward to the Big Bang Fair,  I couldn't even bring a slime…

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Plotting and planning....

Posted on June 2, 2015 at 11:31 0 Comments

Getting involved with several schools in my area running STEM club activities - I'm trying to design a six week course on our dear sticky friend that'll be adaptable to ages 12-17. Looks like it'll be fun ( and a lot of work ). My own cultures have been dormant for a while aside from raising the odd batch but I should start playing again soon. 

Raising slime again..

Posted on May 18, 2015 at 14:17 0 Comments

Blimey it's been a while, we've got the finals lab projects for the students at work on at the moment so I'm not getting time to play. I've got a session with 95 school kids booked in in June so I'm raising as much slime as I can cope with in preparation for that. 

I'm planning on getting them to make Plasticine mazes in petri dishes for their cultures so they've something to take home.

I've also got these intriguing mycena cholorophos spores ( a glowing mushroom ),…

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