How to raise plasmodia from spores - The Slime Mould Collective2024-03-28T21:22:50Zhttps://slimoco.ning.com/forum/topics/how-to-raise-plasmodia-from-spores?commentId=3917201%3AComment%3A47762&feed=yes&xn_auth=no2% agar made up in water. The…tag:slimoco.ning.com,2019-12-13:3917201:Comment:480932019-12-13T06:41:24.933Zianhttps://slimoco.ning.com/profile/ian
<p>2% agar made up in water. The percentage probably doesn't t matter that much but 2% is easier to pick up in forceps than 1</p>
<p>2% agar made up in water. The percentage probably doesn't t matter that much but 2% is easier to pick up in forceps than 1</p> When you say water agar, do y…tag:slimoco.ning.com,2019-12-13:3917201:Comment:481332019-12-13T04:31:09.486ZRyChuhttps://slimoco.ning.com/profile/Raichu
<p>When you say water agar, do you mean agar slices sitting in a pool of water or just standard agar? </p>
<p>When you say water agar, do you mean agar slices sitting in a pool of water or just standard agar? </p> I'm starting to wonder what t…tag:slimoco.ning.com,2019-12-12:3917201:Comment:477922019-12-12T15:38:59.371Zianhttps://slimoco.ning.com/profile/ian
<p>I'm starting to wonder what the hell I was thinking - moving cultures took a couple of hours yesterday but hey, I got to binge Marilyn Manson. All babies now into their own 9cm petri dishes on water agar so I don't have to worry about watering. I think paper strip on top of an agar bed will be the way to do the next one - paper is very handy for moving delicate plasmodia around.…</p>
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<p>I'm starting to wonder what the hell I was thinking - moving cultures took a couple of hours yesterday but hey, I got to binge Marilyn Manson. All babies now into their own 9cm petri dishes on water agar so I don't have to worry about watering. I think paper strip on top of an agar bed will be the way to do the next one - paper is very handy for moving delicate plasmodia around.</p>
<p><a href="https://storage.ning.com/topology/rest/1.0/file/get/3766826089?profile=original" target="_blank" rel="noopener"><img src="https://storage.ning.com/topology/rest/1.0/file/get/3766826089?profile=RESIZE_710x" class="align-center" width="300"/></a>Here's one of the nursery dishes - you can see a little variation in colour but there's far more variation in growth rate so I might have to change selection criteria ( which will be a pita to characterise properly ). On each paper strip there's the tiny snip of agar from the mating dish ( the one with the amoebae on it ) and the one oat it was originally fed. They then got moved onto agar squares and now as some of them are starting to explore they need moving to their own dishes.</p>
<p><a href="https://storage.ning.com/topology/rest/1.0/file/get/3766831696?profile=original" target="_blank" rel="noopener"><img src="https://storage.ning.com/topology/rest/1.0/file/get/3766831696?profile=RESIZE_710x" class="align-center" width="300"/></a></p>
<p>Here's one that did what it was supposed to - move off the paper so I can remove it. You can see just how much contamination there is - the dishes had cm deep rolls of actual moulds growing across them but it's not bothering the slimes ( really cheesing me off though )</p>
<p><a href="https://storage.ning.com/topology/rest/1.0/file/get/3766835251?profile=original" target="_blank" rel="noopener"><img src="https://storage.ning.com/topology/rest/1.0/file/get/3766835251?profile=RESIZE_710x" class="align-center" width="340"/></a>All set up, as soon as the fresh oats are colonised I'll remove the old agar - this is the 'Let the slime outrun the contaminants' strategy.</p>
<p><a href="https://storage.ning.com/topology/rest/1.0/file/get/3766837093?profile=original" target="_blank" rel="noopener"><img src="https://storage.ning.com/topology/rest/1.0/file/get/3766837093?profile=RESIZE_710x" class="align-center" width="340"/></a>Here's one that's a week ahead from a paper test - very active explorer here.</p> Growing away slowly - it seem…tag:slimoco.ning.com,2019-12-09:3917201:Comment:479742019-12-09T07:13:05.861Zianhttps://slimoco.ning.com/profile/ian
<p>Growing away slowly - it seems to take around 4 weeks for them to really pick up. This does work with non sterile conditions but I'm going to start dry toasting the oats - there's a heck of a lot of pin mould and it might not be bothering the slime but it's a massive pain in the arse. </p>
<p>I've also learnt paper is a bad move for small plasmodia - they grow on it fine but there's bugger all water in reserve on a 2cm disc of paper so they dry out easily even when sealed - the water…</p>
<p>Growing away slowly - it seems to take around 4 weeks for them to really pick up. This does work with non sterile conditions but I'm going to start dry toasting the oats - there's a heck of a lot of pin mould and it might not be bothering the slime but it's a massive pain in the arse. </p>
<p>I've also learnt paper is a bad move for small plasmodia - they grow on it fine but there's bugger all water in reserve on a 2cm disc of paper so they dry out easily even when sealed - the water condenses on the petri lid leaving the disc dry. Got around fifty colonies on agar now though and some variation is showing up.</p>
<p></p> Oh no, grains are are already…tag:slimoco.ning.com,2019-11-30:3917201:Comment:480612019-11-30T17:21:34.996Zianhttps://slimoco.ning.com/profile/ian
<p>Oh no, grains are are already crawling with bacteria, it'll be lactic acid and enterobacteria mostly </p>
<p>Oh no, grains are are already crawling with bacteria, it'll be lactic acid and enterobacteria mostly </p> So are you suggesting the oat…tag:slimoco.ning.com,2019-11-29:3917201:Comment:477622019-11-29T22:26:59.007ZRyChuhttps://slimoco.ning.com/profile/Raichu
<p>So are you suggesting the oats be left out for a little bit when you say "Labs use lawns of E.coli, we'll use whatever crap is living on our oats"?</p>
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<p>So are you suggesting the oats be left out for a little bit when you say "Labs use lawns of E.coli, we'll use whatever crap is living on our oats"?</p>
<p></p> One of the excised colonies…tag:slimoco.ning.com,2019-11-29:3917201:Comment:480572019-11-29T08:20:05.025Zianhttps://slimoco.ning.com/profile/ian
<p><a href="https://storage.ning.com/topology/rest/1.0/file/get/3746542035?profile=original" rel="noopener" target="_blank"><img class="align-center" src="https://storage.ning.com/topology/rest/1.0/file/get/3746542035?profile=RESIZE_710x"></img></a></p>
<p>One of the excised colonies after 24 hours - they survive cutting out. The colonies on paper were from multi well plates - too small to use the agar slicing method I used in larger petris - I won't be repeating the multi wells. …</p>
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<p><a href="https://storage.ning.com/topology/rest/1.0/file/get/3746542035?profile=original" target="_blank" rel="noopener"><img src="https://storage.ning.com/topology/rest/1.0/file/get/3746542035?profile=RESIZE_710x" class="align-center"/></a></p>
<p>One of the excised colonies after 24 hours - they survive cutting out. The colonies on paper were from multi well plates - too small to use the agar slicing method I used in larger petris - I won't be repeating the multi wells. </p>
<p><a href="https://storage.ning.com/topology/rest/1.0/file/get/3746546705?profile=original" target="_blank" rel="noopener"><img src="https://storage.ning.com/topology/rest/1.0/file/get/3746546705?profile=RESIZE_710x" class="align-full"/></a></p>
<p>Tiny dot left and slightly down of centre - new plasmodium approx 100 microns, wasn't visible 12 hours before. As far as I understand it the pock marks on the agar are plaques created by feeding amoebae </p>
<p><a href="https://storage.ning.com/topology/rest/1.0/file/get/3746543938?profile=original" target="_blank" rel="noopener"><img src="https://storage.ning.com/topology/rest/1.0/file/get/3746543938?profile=RESIZE_710x" class="align-center"/></a></p>
<p>24 hours after offering a new colony an oat - it's just visible on the bottom right edge</p>
<p><a href="https://storage.ning.com/topology/rest/1.0/file/get/3746544701?profile=original" target="_blank" rel="noopener"><img src="https://storage.ning.com/topology/rest/1.0/file/get/3746544701?profile=RESIZE_710x" class="align-center"/></a></p>
<p>The same oat four days later - growth is slow at this stage</p>
<p><a href="https://storage.ning.com/topology/rest/1.0/file/get/3746550034?profile=original" target="_blank" rel="noopener"><img src="https://storage.ning.com/topology/rest/1.0/file/get/3746550034?profile=RESIZE_710x" class="align-center"/></a></p>
<p>Young plasmodium - maybe 48 hours, TL of fifteen minutes showing it's already streaming.</p>
<p></p> One of the new colony trays -…tag:slimoco.ning.com,2019-11-28:3917201:Comment:477602019-11-28T08:04:52.075Zianhttps://slimoco.ning.com/profile/ian
<p><a href="https://storage.ning.com/topology/rest/1.0/file/get/3745034225?profile=original" rel="noopener" target="_blank"><img class="align-full" src="https://storage.ning.com/topology/rest/1.0/file/get/3745034225?profile=RESIZE_710x"></img></a> One of the new colony trays - the rips of filter paper will hopefully make it easier to move these around - wet filter paper doesn't cut well. JM2 is (Japan x Mazie) selfed on the assumption that outcrossing followed by inbreeding is a sensible way to look for recessive traits and that recessive traits are worth looking for. JM is the only controlled cross…</p>
<p><a href="https://storage.ning.com/topology/rest/1.0/file/get/3745034225?profile=original" target="_blank" rel="noopener"><img src="https://storage.ning.com/topology/rest/1.0/file/get/3745034225?profile=RESIZE_710x" class="align-full"/></a>One of the new colony trays - the rips of filter paper will hopefully make it easier to move these around - wet filter paper doesn't cut well. JM2 is (Japan x Mazie) selfed on the assumption that outcrossing followed by inbreeding is a sensible way to look for recessive traits and that recessive traits are worth looking for. JM is the only controlled cross I've done - picking single spores and culturing is isolation is a massive pain in the bum</p> Thanks
Spent a couple of hou…tag:slimoco.ning.com,2019-11-28:3917201:Comment:480512019-11-28T06:39:04.085Zianhttps://slimoco.ning.com/profile/ian
<p>Thanks </p>
<p>Spent a couple of hours slicing out young plasmodia last night - mm cubes of agar onto paper to minimalise cannibalism. </p>
<p>I was wrong - a supplement did help - mushroom extract ( 10g mushroom boiled 100ml water 3mins strained) one drop added the week after inoculation gave a massive boost to the number of plasmodia</p>
<p>Thanks </p>
<p>Spent a couple of hours slicing out young plasmodia last night - mm cubes of agar onto paper to minimalise cannibalism. </p>
<p>I was wrong - a supplement did help - mushroom extract ( 10g mushroom boiled 100ml water 3mins strained) one drop added the week after inoculation gave a massive boost to the number of plasmodia</p> 11/10 Amazing Work...tag:slimoco.ning.com,2019-11-27:3917201:Comment:479422019-11-27T23:30:52.476ZRyChuhttps://slimoco.ning.com/profile/Raichu
<p>11/10 Amazing Work...</p>
<p>11/10 Amazing Work...</p>