I'm new to slime molds and need help on how to grow them from spores - The Slime Mould Collective2024-03-29T04:53:25Zhttps://slimoco.ning.com/forum/topics/i-m-new-to-slime-molds-and-need-help-on-how-to-grow-them-from?feed=yes&xn_auth=noThat's the way! If you make y…tag:slimoco.ning.com,2022-03-11:3917201:Comment:1579962022-03-11T08:37:23.535Zianhttps://slimoco.ning.com/profile/ian
<p><br/>That's the way! If you make your lighting oblique, you should be able to pick out the trails in the bacterial lawn cleared by the myxamoebae</p>
<p><br/>That's the way! If you make your lighting oblique, you should be able to pick out the trails in the bacterial lawn cleared by the myxamoebae</p> Like this?tag:slimoco.ning.com,2022-03-11:3917201:Comment:1579942022-03-11T03:48:53.190ZMaxwellhttps://slimoco.ning.com/profile/Maxwell158
<p>Like this?</p>
<p>Like this?</p> Just a regular microscope wit…tag:slimoco.ning.com,2022-03-03:3917201:Comment:1580902022-03-03T16:19:06.860ZMaxwellhttps://slimoco.ning.com/profile/Maxwell158
Just a regular microscope with 10x and 25x eyepieces, and 4x, 10x, 40x, and 100x objectives.
Just a regular microscope with 10x and 25x eyepieces, and 4x, 10x, 40x, and 100x objectives. Yep rolled oats. When I say p…tag:slimoco.ning.com,2022-03-03:3917201:Comment:1580892022-03-03T06:41:56.433Zianhttps://slimoco.ning.com/profile/ian
<p>Yep rolled oats. When I say powdered I mean crush it in your fingers.</p>
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<p>By the way, what microscope are you using? </p>
<p>Yep rolled oats. When I say powdered I mean crush it in your fingers.</p>
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<p>By the way, what microscope are you using? </p> Also, I’m a bit confused abou…tag:slimoco.ning.com,2022-03-03:3917201:Comment:1579872022-03-03T00:04:53.410ZMaxwellhttps://slimoco.ning.com/profile/Maxwell158
Also, I’m a bit confused about what types of oats to use. I’m not sure what you meant by “powdered oat flake”. Would rolled oats work, or would I need something even less processed?
Also, I’m a bit confused about what types of oats to use. I’m not sure what you meant by “powdered oat flake”. Would rolled oats work, or would I need something even less processed? Thank you very much! I’ll mak…tag:slimoco.ning.com,2022-03-02:3917201:Comment:1581662022-03-02T22:02:24.951ZMaxwellhttps://slimoco.ning.com/profile/Maxwell158
Thank you very much! I’ll make some adjustments and try this out soon.
Thank you very much! I’ll make some adjustments and try this out soon. Hi
Patience! Myxomycetes are…tag:slimoco.ning.com,2022-03-02:3917201:Comment:1582252022-03-02T16:57:55.223Zianhttps://slimoco.ning.com/profile/ian
<p>Hi </p>
<p>Patience! Myxomycetes are actually quite happy being unicellular - this process takes weeks. </p>
<p>I've only worked with physarum, badhamia and a white thing I couldn't ID successfully though I have Enteridum amoeba on the go at the moment. First thing - don't use instant oats, they've been cooked, have lost some nutrients and crucially for my approach, are less crawling with bacteria. </p>
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<p>Myxamoebae mate when they reach a critical population density, run out of food…</p>
<p>Hi </p>
<p>Patience! Myxomycetes are actually quite happy being unicellular - this process takes weeks. </p>
<p>I've only worked with physarum, badhamia and a white thing I couldn't ID successfully though I have Enteridum amoeba on the go at the moment. First thing - don't use instant oats, they've been cooked, have lost some nutrients and crucially for my approach, are less crawling with bacteria. </p>
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<p>Myxamoebae mate when they reach a critical population density, run out of food or get stressed. They do need a solid surface - at least one has to be in amoebal form - two flagellates can't get it on. </p>
<p>We set up small areas and limited resources with competition from contaminants. Here's my method, you might need to add other things - bark extract and soil extract crop up in papers. With what you have have already you could adapt my protocol and maybe supplement with soil/bark in the agar. You don't have to be exacting - warmish and dampish is fine. </p>
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<p>I've done around 30 repeats of this - </p>
<p>I don't sterilise anything for this and use straight tap water</p>
<p>The culture - crush one sporocarp into 2ml water and add one powdered oat flake. Keep in the dark around 22C for one week. </p>
<p>The agar - 2% water agar in 9cm petris, cut into approx 1cm squares with a few mm gap. </p>
<p>Add few drops of culture - 5 for me, about 25ul to the middle of each square. Don't spread. </p>
<p>Cover and keep dark around 22-24c, plasmodia should start to appear after 3 weeks though 6 is not usual. You'll get a sticky biofilm bug colonies and mould, this is fine. If you look closely at the biolfim you should see patchy areas from feeding myxamoebae </p>
<p>I get about 75% hit rate. Remove and feed plasmodia one crumb of damp oat as soon as you spot (they will cannibalise), you will get contamination at this stage but you can keep the plasmodium moving onto fresh crumbs until it's big enough to subculture. </p>
<p>To give them a nudge you can prop the dish slightly open and let the agar dry and shrink. </p>
<p>Cold shock might be worth a try - just the fridge, these things do follow seasonal pattern after all. </p>
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