Need some help on growing plasmodia and eliminating contamination - The Slime Mould Collective2024-03-28T09:52:31Zhttps://slimoco.ning.com/forum/topics/need-some-help-on-growing-plasmodia-and-eliminating-contamination?commentId=3917201%3AComment%3A158726&x=1&feed=yes&xn_auth=noI too have problems with fung…tag:slimoco.ning.com,2022-05-27:3917201:Comment:1587262022-05-27T20:48:56.446ZKenneth Ramoshttps://slimoco.ning.com/profile/KennethRamos927
<p>I too have problems with fungal hyphae. It seems no sooner than I begin a new moist chamber the mold seems to be waiting. The bumps or tracks that you see on the agar are just that, plasmodia for the most part do leave what I have often read referred to as "slime tracks." I oftentimes see them in my cultures too. As for growth of the plasmodia, only time and nourishment are your best resources in growth. So far one thing I have noted is that <em>P. polycephalum</em> grows in size or seems…</p>
<p>I too have problems with fungal hyphae. It seems no sooner than I begin a new moist chamber the mold seems to be waiting. The bumps or tracks that you see on the agar are just that, plasmodia for the most part do leave what I have often read referred to as "slime tracks." I oftentimes see them in my cultures too. As for growth of the plasmodia, only time and nourishment are your best resources in growth. So far one thing I have noted is that <em>P. polycephalum</em> grows in size or seems too, much faster than <em>P. roseum </em>which I am growing at present. I have a subculture of <em>P. roseum</em> that appears to be taking its good sweet time in both eating and growing and seems to prefer hiding beneath the filter paper. The original plasmodium that I obtained it from appeared right before I was getting ready to throw out a moist chamber culture well over a month old of dogwood tree bark and it has grown to well over 3 to 4 cm in about another month and seems to prefer the standard diet of oat flakes. The subculture is a very picky eater. I don't know if it might be the developing microbial flora that it is waiting on or something of the oat flakes themselves that it likes or doesn't like. All in all its kind of like taking care of a 5 yr. old.</p>