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Shawna
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Very little growth?

Started this discussion. Last reply by ian May 20, 2016. 1 Reply

I'm hoping someone can help me figure out my problem! I got a culture plate from a biological supply company and my subcultures to new plates/environments has not gone super well. There is very…Continue

 

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ian left a comment for Shawna
"I can post out sclerotium for you if you need fresh culture. "
May 20, 2016
ian replied to Shawna's discussion Very little growth?
"Hi It definitely doesn't look very happy, I wonder how long they were in the post for. When they're in the plasmodial stage there's no need to give them E.coli. You could try putting a single moistened ( with water ) oat right on top…"
May 20, 2016
Shawna posted a discussion

Very little growth?

I'm hoping someone can help me figure out my problem! I got a culture plate from a biological supply company and my subcultures to new plates/environments has not gone super well. There is very little growth. I have grown them on 2% agar, moistened paper towel, recently tried some on dampened filter paper on top of 2% agar, corn meal agar. Feeding them has included sterile oats, non-sterile oats, OP50 e. Coli (the kind they feed to c. Elegans), glucose, and then combos of oats moistened with e…See More
May 19, 2016
Shawna is now a member of The Slime Mould Collective
May 19, 2016

Profile Information

Name
Ohlmann
Interest in slime moulds
Academic
Academic Institution (if applicable)
MacEwan

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At 11:46 on May 20, 2016, ian said…

I can post out sclerotium for you if you need fresh culture. 

 
 
 

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