The Slime Mould Collective

An international network of/for intelligent organisms

Zander Chierici
  • San Mateo, CA
  • United States
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Any tips on finding an affordable camera making slime time lapses?

Started this discussion. Last reply by ian Jul 20. 3 Replies

I work at a highschool lab (as a passionate student) and am designing slime mould experiments for next year's biology class. I have been trying to find a good camera for capturing slimes in low or or…Continue

Tags: timelapse, Physarum_polycephalum

 

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ian replied to Zander Chierici's discussion Any tips on finding an affordable camera making slime time lapses?
"I'm using the standard kit lenses - 18-55mm, about 40cm above the tray you can get a 30x30cm field of view, I run assays either on a whole plate of agar or on 5x5cm agar squares with a paper top ( eliminates problems with shrinkage ). I used to…"
Jul 20
Zander Chierici replied to Zander Chierici's discussion Any tips on finding an affordable camera making slime time lapses?
"I'm not too familiar with canon cameras, do you have to have a lense to get good photos of the slime? If so, what size lense do you use?"
Jul 12
ian replied to Zander Chierici's discussion Any tips on finding an affordable camera making slime time lapses?
"Hi I use Canon eos-m's - fairly cheap second hand and they run magic lantern so you can time lapse with long exposure. If you're thinking of Ir just to keep slimes happy, Amber leds are a lot easier - physarum can't see light…"
Jul 4
Zander Chierici posted a discussion

Any tips on finding an affordable camera making slime time lapses?

I work at a highschool lab (as a passionate student) and am designing slime mould experiments for next year's biology class. I have been trying to find a good camera for capturing slimes in low or or infrared light, but since it's a high school class the budget is about $2000 for 5 cameras. But would prefer to stay on the cheap side if possible.Does anybody have any camera recommendations or infrared set ups? I was thinking of taking out the infrared filter in my gopro and adding an infrared…See More
Jul 1
Zander Chierici updated their profile
Jun 30
Zander Chierici is now a member of The Slime Mould Collective
Jun 28

Profile Information

Name
Zander
What is your interest in slime moulds? (we need to know you're not a bot or spamster)
I am helping create/design a slime mold (mould?) project for Bio 1 students in high school.
Here are the list of goals for improving the project:

1. A more efficient and fool-proof system for growing/maintaining slime mold
a. How to start from sclerotium
b. How to maintain a growing culture and minimize contamination
c. How to get uniform “starts” from a growing culture
2. An iterated and well-tested set of assays and materials/tools for experimentation
a. Best versions of choice assays, bridge assays, maze assays, U-shaped trap, etc
b. Ideal concentrations, solutions, and means of employing both attractants and repellents.
3. A group of easy-to-implement measurement protocols and tools
a. Time lapse rig set-ups and analysis
b. Best practices for measurements w/o time lapse
c. Measuring growth in different ways:
d. Surface area, path lengths, rate, etc.
e. Still image analysis:
f. Image J/Fiji or explore other programs

I am really excited to learn more about slime molds through this project and I hope to use the Slime Mould Collective as a valuable resource for questions and problems I encounter.
Academic Institution (if applicable)
The Nueva School
Website
http://https://www.nuevaschool.org/

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