The Slime Mould Collective

An international network of/for intelligent organisms

Miguel Grande
  • Oxford
  • United Kingdom
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Miguel Grande's Discussions

Physarum onto glass?

Started Dec 2 0 Replies

Hi everyone,I’m currently working with Physarum polycephalum and I have a question regarding substrates.For my research, I need the plasmodium to be in direct contact with a glassy surface (a smooth,…Continue

Physarum always dies

Started this discussion. Last reply by qiuwenxing Dec 1. 2 Replies

Hello everyone,I recently bought Physarum polycephalum from Carolina and I’ve been trying to grow it from sclerotia into plasmodium. I’ve attempted this several times. Usually it wakes up and looks…Continue

 

Miguel Grande's Page

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Miguel Grande posted a discussion

Physarum onto glass?

Hi everyone,I’m currently working with Physarum polycephalum and I have a question regarding substrates.For my research, I need the plasmodium to be in direct contact with a glassy surface (a smooth, non-porous material). I was wondering if Physarum can be successfully cultured on glass.My current idea is the following:take an active, healthy plasmodium,place it upside down so that the living side is touching the glass surface directly,and then place a small moist piece of paper with an oat…See More
Dec 2
qiuwenxing replied to Miguel Grande's discussion Physarum always dies
"you can also keep it on the paper."
Dec 1
qiuwenxing replied to Miguel Grande's discussion Physarum always dies
"I support the view of bacterial contamination because sterile culture under home conditions is impossible, and slime molds themselves also carry symbiotic bacteria. I suggest using better agar or switching to agarose (note not to introduce…"
Dec 1
Miguel Grande posted a discussion

Physarum always dies

Hello everyone,I recently bought Physarum polycephalum from Carolina and I’ve been trying to grow it from sclerotia into plasmodium. I’ve attempted this several times. Usually it wakes up and looks healthy (bright yellow the next day), but then it suddenly stops growing or dies.At first, I suspected contamination. My early attempts were done in a hood but without sterilizing the oats or the water. In those cases, the Physarum initially grew well but then dried out or decomposed after a few…See More
Dec 1
Miguel Grande is now a member of The Slime Mould Collective
Nov 28

Profile Information

Name
Miguel
What is your interest in slime moulds? (we need to know you're not a bot or spamster)
I want to grow them for research purposes
Academic Institution (if applicable)
University of Oxford
Complete the phrase from the website title bar - "An international network of/for "....... Amusing answers will be accepted
intelligent organisms

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