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Christian Diwo
  • Kuala Lumpur
  • Malaysia
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Christian Diwo's Discussions

negative stain TEM images of sheared physarum protoplasmic tubes!?

Started Mar 16, 2018 0 Replies

Hey,I did my master thesis in structural biology, we imaged the cytoskeleton of red blood cells. I had some spare time and tried the same method with physarum (kindly provided by Ian). Not sure what…Continue

Tags: cytoskeleton, stain, negative, TEM

Light filter that would allow to keep cultures in normal light conditions

Started this discussion. Last reply by Dr Jason bell Dec 9, 2017. 7 Replies

Hello everyone,I did not start cultivating physarum yet, but my cultures will arrive soon and I am about to set up my lab.I was wondering if anybody knows about experiments done with light filters…Continue

 

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Christian Diwo posted a discussion

negative stain TEM images of sheared physarum protoplasmic tubes!?

Hey,I did my master thesis in structural biology, we imaged the cytoskeleton of red blood cells. I had some spare time and tried the same method with physarum (kindly provided by Ian). Not sure what we see on the images, but I wanted to share them anyway. There might be nuclear pore complexes... hope its interesting for someone!See More
Mar 16, 2018
Dr Jason bell replied to Christian Diwo's discussion Light filter that would allow to keep cultures in normal light conditions
"We are about to start several experiments with light. I will keep this updated, the other experiment might require help of people here. We would like to sequence population genomes, collecting some wild specimens has thrown up some unusual results."
Dec 9, 2017
Jasmin Marker replied to Christian Diwo's discussion Light filter that would allow to keep cultures in normal light conditions
"I know this is an old post , but has anybody done anymore experiments? I came across this paper where they found that blue light fragments the slimemold. When a plasmodium is illuminated by ultraviolet or blue monochromatic light it breaks up into…"
Nov 18, 2017
ian replied to Christian Diwo's discussion Light filter that would allow to keep cultures in normal light conditions
"They really hate UV or anything down the blue end of the spectrum. They can't see their own colour so you could use it for lighting, the three pigments that make physarum yellow are soluble in alcohol and acetone so you could actually grow up a…"
Jan 27, 2017
François-Joseph Deminiere replied to Christian Diwo's discussion Light filter that would allow to keep cultures in normal light conditions
"Hi, Did you try diffrent led color? I'm interested too !"
Jan 19, 2017

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