An international network of/for intelligent organisms
To curb Physarum Polycephalum's (for convenience known as Andi in our household) tendency to wander, I have used a 'moat', a ring of high-salt agar around its standard agar living area. This works fine, only is somewhat labor intensive to maintain and also harms Andi, who keeps burning itself trying to go where no slime mold went before.
I experimented with four alternative substrates to replace the salty agar: 1. felt, thinking this would interfere with its creeping motion; 2. cardboard, which would dry it out; 3. Aluminum, interfering with some electrochemical process or other; and 4. rubber, for no clear reason whatsoever but I like symmetry.
Initially, it was reluctant to crawl on the felt or aluminum, but had no problem with rubber or cardboard, although the cardboard indeed drained it quite a bit. Next, I extended the oatmeal it likes to eat onto the alternative substrates.
Ah, sporulation on the cardboard floor, probably initiated by drying out.. although in another experiment, drying it out by a slightly elevated temperature caused it to form a sclerotium, so there must be other factors at play too! Ultimately, the surface least explored was aluminum, so in the next container I am building, I will add a ring of aluminum to keep it contained. Here are some detail pictures of Andi on various substrates, the last picture shows salt burns caused by the earlier enclosure:
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