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Small plasmodia that are large enough to stream, alongside empty spore cases and hatched amoebas.
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There's this classic: http://jcs.biologists.org/content/107/8/2071.full.pdf Actually, re-reading this one makes me think that the big 'spores' in the video are actually cysts. I've tried cAMP on the plasmodium and it has very little reaction, perhaps it's still secreting it to the amoebas though?
I'd put money on cAMP being involved, might do a Friday afternoon literature trawl after my real work's done. Are these guys able to divide during their free living phase? I've never found an answer to that question
On agar with E. coli B/r. Catching fusion would be amazeballs. I swear I can see amoebas trying desperately to fuse with already-fused tiny plasmodia. I wonder if there's a 'fusion-signal' in a sub-population of cells??
Were these guys in water or on agar?
I'd love to catch the moment that two cells fuse and start to form a plasmodium, something tells me that might be quite difficult to achieve though.
Thanks. I'm not sure if uploading to youtube messes around with image size but there is a little scale bar for 20 microns in the bottom right. The larger plasmodium is about 400 microns tall. This field of view should be similar with a 20x lens on an upright microscope.
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