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Maxwell
  • Elk Grove, CA
  • United States
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Maxwell's Discussions

Sporulation?

Started May 26, 2023 0 Replies

     Recently, I’ve been messing around with a hemitrichia plasmodium that I grew from sporangia obtained from the wilderness. The plasmodia was white at first, but began to turn yellow after it…Continue

Getting Plasmodia to sporulate and form sclerotia

Started Apr 19, 2023 0 Replies

Hello, I’ve been trying to complete the spore to spore life cycle of a hemitrichia species. I’ve had success in getting it form plasmodia, but I’d like some advice past this point. I’d like to know…Continue

Help with myxamoeba encystment and plasmodia formation

Started this discussion. Last reply by ian Sep 8, 2022. 1 Reply

Recently, I’ve been trying to grow hemitrichia sp. and stemonitis spp. from spores harvested from the wild. I’ve had success in getting the spores to germinate, but I haven’t been able to get the…Continue

Need some help on growing plasmodia and eliminating contamination

Started this discussion. Last reply by Kenneth Ramos May 27, 2022. 1 Reply

Hello. Recently I’ve been trying to grow some unidentifiable plasmodium on some water agar plates. I obtained this plasmodium by finding it under a decaying log on a hike. Since all plasmodia look…Continue

 

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Maxwell posted a discussion

Sporulation?

     Recently, I’ve been messing around with a hemitrichia plasmodium that I grew from sporangia obtained from the wilderness. The plasmodia was white at first, but began to turn yellow after it began depleting the agar on which I was growing it on. I then transferred it to a larger plate, and after a couple of days, I began noticing some weird behavior. It had originally spread out across the plate, but was now clumping up into mounds which had the appearance of scrambled eggs (Picture 1). The…See More
May 26, 2023
Maxwell posted a photo
Apr 28, 2023
Maxwell posted a discussion

Getting Plasmodia to sporulate and form sclerotia

Hello, I’ve been trying to complete the spore to spore life cycle of a hemitrichia species. I’ve had success in getting it form plasmodia, but I’d like some advice past this point. I’d like to know how to get the plasmodia to form sclerotia and sporulate. Frim what I know, sporulation is encouraged when the slime mold runs out of food and is exposed to light, and they form sclerotia when dehydrated. I have a couple questions regarding this. Firstly, does would any light work getting them to…See More
Apr 19, 2023
Maxwell updated their profile
Apr 19, 2023
Maxwell posted photos
Apr 19, 2023
ian replied to Maxwell's discussion Help with myxamoeba encystment and plasmodia formation
"Hi Feed them bugs! the myxamoeba like a lawn of bacteria to feed off - I make a suspension of 1 oat per ml in water, let it sit a few days and pipette about 50 microlitres onto the dish when I add spores.  It can takes weeks to months for…"
Sep 8, 2022
Maxwell posted a discussion

Help with myxamoeba encystment and plasmodia formation

Recently, I’ve been trying to grow hemitrichia sp. and stemonitis spp. from spores harvested from the wild. I’ve had success in getting the spores to germinate, but I haven’t been able to get the amoeba to form plasmodia. Another problem that I’ve been having is that a couple days after germinating, all of the myxamoeba in the culture will randomly encyst. This may be why I haven’t had luck getting plasmodia as the amoeba don’t stay amoeba long enough to form one, but I’m not entirely sure.…See More
Sep 6, 2022
Kenneth Ramos replied to Maxwell's discussion Need some help on growing plasmodia and eliminating contamination
"I too have problems with fungal hyphae.  It seems no sooner than I begin a new moist chamber the mold seems to be waiting.  The bumps or tracks that you see on the agar are just that, plasmodia for the most part do leave what I have often…"
May 27, 2022
Maxwell posted a discussion

Need some help on growing plasmodia and eliminating contamination

Hello. Recently I’ve been trying to grow some unidentifiable plasmodium on some water agar plates. I obtained this plasmodium by finding it under a decaying log on a hike. Since all plasmodia look similar, I wasn’t able to identify it without being able to see its fruiting body. You can see the original plasmodium I found in the second picture. Anyways, I’ve had some trouble with actually growing it. If you look really closely around the central agar cube in the first photo, you can see what…See More
May 27, 2022
ian replied to Maxwell's discussion I'm new to slime molds and need help on how to grow them from spores
"That's the way! If you make your lighting oblique, you should be able to pick out the trails in the bacterial lawn cleared by the myxamoebae"
Mar 11, 2022
Maxwell replied to Maxwell's discussion I'm new to slime molds and need help on how to grow them from spores
"Like this?"
Mar 11, 2022
RyChu liked Maxwell's discussion I'm new to slime molds and need help on how to grow them from spores
Mar 3, 2022
Maxwell replied to Maxwell's discussion I'm new to slime molds and need help on how to grow them from spores
"Just a regular microscope with 10x and 25x eyepieces, and 4x, 10x, 40x, and 100x objectives."
Mar 3, 2022
ian replied to Maxwell's discussion I'm new to slime molds and need help on how to grow them from spores
"Yep rolled oats. When I say powdered I mean crush it in your fingers. By the way, what microscope are you using? "
Mar 3, 2022
Maxwell replied to Maxwell's discussion I'm new to slime molds and need help on how to grow them from spores
"Also, I’m a bit confused about what types of oats to use. I’m not sure what you meant by “powdered oat flake”. Would rolled oats work, or would I need something even less processed?"
Mar 3, 2022
Maxwell replied to Maxwell's discussion I'm new to slime molds and need help on how to grow them from spores
"Thank you very much! I’ll make some adjustments and try this out soon."
Mar 2, 2022

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Name
Maxwell
What is your interest in slime moulds? (we need to know you're not a bot or spamster)
I’ve been collecting slime mold sporocarps from the California redwoods in the hope of completing the spire to spite life cycles of various species. So far, I’ve had great success in getting a hemitrichia species to the plasmodium stage. I’ve also gotten a couple other species to form myxamoeba.

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