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Hello, I wanted to ask few questions about slime mold living conditions:
I grow Ph. Polycephalum at room temperature, but it will grow at lower temperatures just fine, just slower.. if I were in the opportunity to provide a specific temperature, I would look at the geographical distribution and life cycles of these organisms, and take my cue from there. Maybe nobody looked at this yet, and you can do some interesting experiments?
If you sterilize the agar, boil oats in a pressure cooker, and plug the air hole of your enclosure with cotton wool, you delay the onset of white fungus and other opportunists. Slime molds do need a damp environment though and will form a sclerotium, or change into the sporulating phase of their life cycle if things get too dry.