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I am currently attempting to culture physarum polycephalum directly onto 3D printed surfaces without the application of agar. I have had luck previously creating cultures on agar coated surfaces, but I can achieve a higher resolution without the coat and have spoken with researchers who have claimed to accomplish this.
Environment: enclosure is kept at 70% humidity, 72 degrees, and contains a HEPA air filter for circulation and decontamination. Light is low, it's in a basement and the enclosure is walled off.
Growth surfaces: 4"x4" tiles 3D printed on a Formlabs Form 2 with white resin, decontaminated according to biocompatibility protocols used for bacterial growth (two cycles in 90% isopropyl alcohol, full drying, UV curing). The surfaces are sterilized with 70% enthanol and allowed to dry before colonized oats are added onto the surface with sterilized tweezers.
Biology: Species is physarum polycephalum, food targets are unclaved raw oats. I maintain a mother culture on an agar surface and have had luck transferring to other agar plates as well as paper towels, but not to plastic.
Blooming: The HEPA filter puts off a faint blue light. I have largely obscured it, but one of the cultures I was growing on paper towels began to bud after two days. I have heard that this happens in response to UV light exposure. Any advice on preventing this behavior would also be appreciated.
I was advised to leave a layer of water below the printed surfaces to improve the humidity levels in my enclosure. I seeded new growth today and am hoping this helps.
Additionally, if anybody has experience taking photos of physarum growth over time, I would appreciate the help.
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