"The sporangia are well formed as are the rest of the fruitbody components and spores are released but a dust cloud is seldom noticed but only on occasion and during observation through the dissecting microscope and slide preparation for observing…"
An international network of/for intelligent organisms
Vicente Lopes's Discussions
Sporulation of Physarum
Started this discussion. Last reply by Kenneth Ramos Jan 18. 5 Replies 0 Likes
I've tried quite a bit of sporulation induction for my Physarum culture since I need spores for experiments that I plan to do.However, every time I try inducing the sporulation it appears to die…Continue
Vicente Lopes's Page
Latest Activity
"and in your cultures do your sporangia release spores into the air, like a dust cloud?
I assume this is a hallmark to know if the sporangia are well formed and viable for germination..."
"I keep the plasmodia in the dark, with the exception of removal for observation with the dissecting microscope. Using moist chamber cultures, petri dishes (plastic or borosilicate) with dampened paper towel and oat flakes. I don't…"
"By "induced sporulation" i mean i basically tried to slowly starve it (less food than needed) in light conditions, after different setups this seems to be the best at causing sporulation (within 7 days) however it never seems to finish…"
"What is it that you mean by "induced sporulation?" Sporulation is a touchy process. As you probably already know, temperature, humidity, and light among the most prominent are major players in getting a plasmodium to…"
Sporulation of Physarum
I've tried quite a bit of sporulation induction for my Physarum culture since I need spores for experiments that I plan to do.However, every time I try inducing the sporulation it appears to die while sporulating, leading to immature sporangia.After crushing the sporangia some spheres are visible (maybe spores) but they don't come out of the sporangia naturally (no dust) and they don't germinate.Here is a micro photo of the crushed sporangia with spore like spheres, and deformed sporangia in…See More
"I've tried it before and had no luck unfortunately where did you collect the bark? In a forest or a more suburban environment?"
"Hi Dan,
Just sent the e-mail, let me know if you got it!
Vicente"
"Here is one of the samples.
I have 12 photos of different colonies, I think I was a bit late so maybe 1 day of growth is better than 2 days. Let me know how I can send the other photos and what you think.
Vicente"
"I've currently started 12 circular colonies with oats spread out in varying random and geometric presets (triangle, square, pentagon, etc...).
Later this week, I'll photograph them once they look good and developed!
Vicente"
"Hi Dan,
I'm willing to help with the source material!
Let me know the specifications you might have in mind."
"Hi,
In case it's not too late, I have a 3d printer so if you have a specific 3d model of a maze I can print it and send it by post.
Cheers
Vicente "
"Hallo,i'mlooking for a Physarum culture since whatigot fromaseller i identified it as Badhamia... Any europeans that can share lmk! Aus Koln"
Profile Information
- Name
- Vicente
- What is your interest in slime moulds? (we need to know you're not a bot or spamster)
- I'm interested in the slime mold group in general, particularly in the adaptability and aging-related changes of physarum.
- Academic Institution (if applicable)
- University of Cologne, Germany
- Complete the phrase from the website title bar - "An international network of/for "....... Amusing answers will be accepted
- intelligent boogers
© 2025 Created by Heather Barnett.
Powered by
