Searching and collecting wild slime molds Part 2 - Slime mold Extravaganza!
In this video, my slime mold sense has definitely improved since the previous episode!We hit absolute gold, the color at least! I found a ton of slime molds ...
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Vicente Lopes's Discussions
Sporulation of Physarum
Started this discussion. Last reply by Vicente Lopes Feb 4. 6 Replies 0 Likes
I've tried quite a bit of sporulation induction for my Physarum culture since I need spores for experiments that I plan to do.However, every time I try inducing the sporulation it appears to die…Continue
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Searching and collecting wild slime molds! I swear i find some in the end :P
This is the first long-format video of the channel, it was very fun to film and edit!Here I try searching for slime molds in the forest and have fun also fin...
10 relaxing minutes of slime molds under the microscope (turn on captions)
I've been working on a microscope setup to film slime molds up close and felt that the footage could make a very relaxing and interesting video. Similar to w...
Fireworks after feeding!
Added a timer with the hours passing to give a better time perspective! Also cool explosion effect after feeding! First time I see this type of behaviour tim...
Slime mold finding and consuming Oat flakes
In the first video of the channel, I documented the movement and behaviour of my Physarum polycephalum slime mold.Just trying the formats and hopefully find ...
"Thats crazy dude.
Wishing you the best"
"Update:
I think i finally achieved finished sporangia. The sporangia are a lot larger and more complex than anything I've gotten before and after a bit of time the individual sporangium turned from a moist/shiny black to a dull grayish colour.…"
"The sporangia are well formed as are the rest of the fruitbody components and spores are released but a dust cloud is seldom noticed but only on occasion and during observation through the dissecting microscope and slide preparation for observing…"
"and in your cultures do your sporangia release spores into the air, like a dust cloud?
I assume this is a hallmark to know if the sporangia are well formed and viable for germination..."
"I keep the plasmodia in the dark, with the exception of removal for observation with the dissecting microscope. Using moist chamber cultures, petri dishes (plastic or borosilicate) with dampened paper towel and oat flakes. I don't…"
"By "induced sporulation" i mean i basically tried to slowly starve it (less food than needed) in light conditions, after different setups this seems to be the best at causing sporulation (within 7 days) however it never seems to finish…"
"What is it that you mean by "induced sporulation?" Sporulation is a touchy process. As you probably already know, temperature, humidity, and light among the most prominent are major players in getting a plasmodium to…"
Sporulation of Physarum
I've tried quite a bit of sporulation induction for my Physarum culture since I need spores for experiments that I plan to do.However, every time I try inducing the sporulation it appears to die while sporulating, leading to immature sporangia.After crushing the sporangia some spheres are visible (maybe spores) but they don't come out of the sporangia naturally (no dust) and they don't germinate.Here is a micro photo of the crushed sporangia with spore like spheres, and deformed sporangia in…See More
"I've tried it before and had no luck unfortunately where did you collect the bark? In a forest or a more suburban environment?"
"Hi Dan,
Just sent the e-mail, let me know if you got it!
Vicente"
"Here is one of the samples.
I have 12 photos of different colonies, I think I was a bit late so maybe 1 day of growth is better than 2 days. Let me know how I can send the other photos and what you think.
Vicente"
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