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I've tried quite a bit of sporulation induction for my Physarum culture since I need spores for experiments that I plan to do.
However, every time I try inducing the sporulation it appears to die while sporulating, leading to immature sporangia.
After crushing the sporangia some spheres are visible (maybe spores) but they don't come out of the sporangia naturally (no dust) and they don't germinate.
Here is a micro photo of the crushed sporangia with spore like spheres, and deformed sporangia in the right (looks like they couldn't finish developing).
Is this normal? Should I just keep doing it until they form properly? Tips are welcomed.
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