"I don't have any specimens on hand but I used the rough bark of a Flowering Dogwood to prepare moist culture chambers. Other substrates may be just as productive. Incubation time was about 21 days for P. roseum. Soak the bark…"
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Kenneth Ramos
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IMG_0099 P. roseum
"Do you still keep roseum? If so, you wouldn't happen to have extra sclerotia would you?"
BlueMtnStone - (85)-HDRaRed
"Most anything from the Permian fascinates me because so little survived from it towards the end. These could easily be mistaken for slime mold plasmodia I suppose but more along the line of an alga of sorts or maybe a fern of some kind. …"
"Microsoft PowerPoint - IsoHandbook.2 (uark.edu)
Culturing slime molds from the environment. Hope this helps you get started."
IMG_101@45X P. roseum plasmodium
Though amoeboid movement in Physarum is not unusual or uncommon, it is quite self evident, I was delighted to see it in progress other than the usual cytoplasmic streaming we see in the veinous cytoplasm normally. While intently observing P. roseum…
thumbnail_IMG_8821
"Personally I don't know of any slime mold fruit bodies showing up in the fossil record other than in amber maybe, along with spore or two. You present some very good questions though, ones that I wish I could knowledgeably discuss…"
"Try using a dry cell and two electrodes one (+) the other (-) of course and place them at opposite sides of the petri dish. Attach the battery or dry cell and observe what happens. A single AA battery should suffice, if not try a D…"
"I too have problems with fungal hyphae. It seems no sooner than I begin a new moist chamber the mold seems to be waiting. The bumps or tracks that you see on the agar are just that, plasmodia for the most part do leave what I have often…"
IMG_0099 P. roseum
P. roseum, circle the wagons, it's chow time. Oatflake in moist chamber, petri dish.
IMG_0094 Physarum roseum
"Most of the Physarum I have obtained through moist chamber cultures have all been yellow in color, however, I read where the color can sometimes be due to the pH of the substrate on which the plasmodium resides. Again, however,…"
IMG_0094 Physarum roseum
Physarum roseum, obtained from Flowering dogwood tree bark in moist chamber culture.
"Why are they adverse to light? Good question, I am not sure of that myself but reading your post had me looking at positive and negative phototaxis and how it plays out in prokaryotic and eukaryotic organisms. I did not quite find…"
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- Ken
- What is your interest in slime moulds? (we need to know you're not a bot or spamster)
- Citizen scientist, amateur naturalist and researcher who studies Eumycetozoans among others. Currently my studies are of corticolous myxomycetes, fungi and other tree bark-dwelling organisms. Other interests include Paleontology, Geology, Mass extinctions, Evolution and environmental science
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