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Cire Sniknej
  • Logan, UT
  • United States
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How do you grow a P. Polycephalum from spores?

Started this discussion. Last reply by ian Jan 31. 6 Replies

I currently have two slime molds going, and have one that is making sporangia, so I was wondering how to grow a slime mold from the spores. Do you feed it oats, or what?Continue

 

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ian replied to Cire Sniknej's discussion How do you grow a P. Polycephalum from spores?
"Chalk might be worth a try - used to use it in the lab to bulk up lactobacilli by adding it to skimmed milk and culture. Depends on how much they like (or don't) Calcium."
Jan 31
ian replied to Cire Sniknej's discussion How do you grow a P. Polycephalum from spores?
"I think there'd be too much crap in there from the curdled milk and slime really doesn't like sodium ions much. Could be worth a try diluted though."
Jan 31
François-Joseph Deminiere replied to Cire Sniknej's discussion How do you grow a P. Polycephalum from spores?
"Hi ian, Can't we simply use a Yogurt (or so) for it's dead bacteria, and just ad baking soda to neutralize remaining lactic acid from serum (test with ractive paper) ? FJD"
Jan 31
ian replied to Cire Sniknej's discussion How do you grow a P. Polycephalum from spores?
"Thinking about about this last night ( I'm a world class insomniac).  The bacteria don't need to be alive and good old coli isn't a great choice if you're not in a lab - it smells awful for one thing.  I wonder if lacto…"
Jan 31
Cire Sniknej replied to Cire Sniknej's discussion How do you grow a P. Polycephalum from spores?
"thanks!"
Jan 30
ian replied to Cire Sniknej's discussion How do you grow a P. Polycephalum from spores?
"The spores need to dry for a few weeks and will then hatch into amoebae if you add water.  The haploid ameobae feed off bacteria I've grown them up in liquid culture by adding spores to a suspension of E.coli - not really practical unless…"
Jan 30
Cire Sniknej is now a member of The Slime Mould Collective
Dec 1, 2016

Profile Information

Name
Cire
Interest in slime moulds
I wand to try to keep one for a while

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