The Slime Mould Collective

An international network of/for intelligent organisms

ian
  • Birmingham
  • United Kingdom
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Ian's Friends

  • Irina Antonets
  • Raichu
  • Blob Morane
  • Evan Brown
  • Gaspard Bébié-Valérian
  • mint taste slime mould
  • DONGYUANLI
  • Lauren Loop
  • Kendra Fanconi
  • Kornelija
  • Sarah
  • Patrick Barry
  • Malwina Kotowicz
  • Josh Evans
  • ines tavernier

ian's Discussions

Incubator design

Started Nov 28 0 Replies

Using an aquarium heater (100w) in a 50l plastic tub was a disaster - condensation, puddles of water, stinks and doesn't heat the box.The mk 2 - reptile heating pad ( 50w) sandwiched between a slab…Continue

How to raise plasmodia from spores

Started this discussion. Last reply by ian on Saturday. 6 Replies

The results are coming in from my first trials and it looks like all the other methods aren't necessary.This has a 70% hit rate, faster development would be nice but this works.Some repetition here…Continue

Under the electron microscope

Started this discussion. Last reply by Nemo Andrea Nov 23. 5 Replies

Nice thing about managing a lab is sometimes you get to have a bit of a play. We had clinical samples to process the other week and also a lab full of slime moulds left over from filming. The…Continue

Starting from spores - todays trials

Started this discussion. Last reply by ian Nov 17. 2 Replies

Another trial run. I've 'built' an 'incubator' - a 1960's paper says warmth is crucial so I've put an aquarium heater in a tank of water inside a large tub, it's set to 28C.I'm trying many methods at…Continue

 

ian's Page

Latest Activity

ian replied to Raichu's discussion Foraging 360 degrees
"If you're gonna do that, make sure you scrape out the remains of the old food before adding more or it'll start to rot. They'll go about a week like that before they need fresh bedding - I fold the culture in half and put it on the…"
yesterday
ian replied to Raichu's discussion Foraging 360 degrees
" This one was scelrotium on 30th November, pic taken this morning - they grow fast if you let them "
yesterday
ian replied to Raichu's discussion Foraging 360 degrees
"You're not feeding your slime enough; start with about ten oats, feed as soon as it's covered most of its existing food. Double the feed every time. On day five I'm using 50-100g of oats - twice a day per dish, ok so I'm making a…"
Tuesday
ian replied to Raichu's discussion Foraging 360 degrees
"Yeh I typically use a teaspoon to tablespoon sized blob to start things off - particularly on maps"
Monday
ian replied to Raichu's discussion Foraging 360 degrees
"Time, size and luck basically - that's a large starting piece in the middle so it's got the luxury of having enough biomass to explore in multiple directions. If it doesn't find food quickly it's more likely to start off another…"
Monday
ian replied to ian's discussion How to raise plasmodia from spores
"Oh no, grains are are already crawling with bacteria, it'll be lactic acid and enterobacteria mostly "
Saturday
ian replied to ian's discussion How to raise plasmodia from spores
"One of the excised colonies after 24 hours - they survive cutting out. The colonies on paper were from multi well plates - too small to use the agar slicing method I used in larger petris - I won't be repeating the multi wells.  Tiny dot…"
Nov 29
ian replied to William's discussion I'm looking for Blob
"Take home message - it might be worth feeding viagra to physarum...."
Nov 28
ian replied to William's discussion I'm looking for Blob
"Daywalker is P.polycephalum ( the only non physarum I distribute is Mandy the presumed cribraria ). I don't know if it's 'blind' ( ie can't detect light ) or sporulation defective - experiment to be set up tonight ( time…"
Nov 28
ian replied to ian's discussion How to raise plasmodia from spores
"One of the new colony trays - the rips of filter paper will hopefully make it easier to move these around - wet filter paper doesn't cut well. JM2 is (Japan x Mazie) selfed on the assumption that outcrossing followed by inbreeding is a sensible…"
Nov 28
ian posted discussions
Nov 28
ian replied to Raichu's discussion Slime and Iodine
"You can buy caffeine over the counter in pill form - there's lactose in there as well so you might need another control but as you need milligrams of caffeine it might be easier that the pure substance "
Nov 28
ian replied to ian's discussion How to raise plasmodia from spores
"Thanks  Spent a couple of hours slicing out young plasmodia last night - mm cubes of agar onto paper to minimalise cannibalism.  I was wrong - a supplement did help - mushroom extract ( 10g mushroom boiled 100ml water 3mins strained) one…"
Nov 28
ian replied to William's discussion I'm looking for Blob
"I rear strains now and then from spores, Daywalker was just one that cropped up with a noticeably different trait. I have a hybrid ( presumed hybrid anyway - I haven't done genetics on it ) between the Hokkaido ( Japan ) strain and…"
Nov 27
ian replied to William's discussion I'm looking for Blob
"Just because these cultures came from labs in different countries it doesn't mean they originated in them - they've been around since the 70's and I don't think anyone knows where they came from but I have strains of Physarum…"
Nov 27
ian replied to Raichu's discussion Please Help - Sporulation Occured
"Hi Your cultures is doomed - unless some part of it is still yellow and crawling it's well dead. As Evan's said the spores are dormant - takes around 3 weeks of being dry for them to activate. It's light exposure - once a plasmodium…"
Nov 27

Profile Information

Name
hardboiledparrot
What is your interest in slime moulds? (we need to know you're not a bot or spamster)
I'm a professional microscopist and amateur time lapse filmer. I'm looking forward to combining both disciplines with these fascinating organisms
Academic Institution (if applicable)
warwick
Website
http://www.youtube.com/channel/UC5ldTLqKM6QuRQ7zaimMdxg

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Comment Wall (6 comments)

You need to be a member of The Slime Mould Collective to add comments!

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At 15:36 on October 10, 2019, Sean said…
Hello! Thank you for the warm welcome! I’m excited to get working with some slime mould. Just spent the day hunting/photographing some in Epping forest but pretty sure I got 95% mushrooms. Any chance I could send you some pictures of what I think are slime mould spore capsules for your opinion?
At 21:03 on January 16, 2018, Kendra Fanconi said…

Oh that would be amazing. I was looking at ordering it from the US. Is there any issue with getting it through customs? 

At 16:05 on October 27, 2015, Marijn Roelvink said…
We received the Physarum today, thanks a lot!
At 12:19 on October 14, 2015, Marijn Roelvink said…
Hi Ian, thank you for wanting to send the Physarum, I was just wondering if you could tell me when they will arrive, it'll help a lot with our planning :)
At 10:37 on February 10, 2015, enrico viola said…
I received my samples today: thanks a lot!
At 8:10 on February 3, 2015, enrico viola said…

Sent you a pm with my address; thanks again!

Ian's Blog

Raising from Spores - Zero Tech Method

Posted on April 11, 2017 at 13:30 0 Comments

I've been looking for a way to rear physarum from spores without resorting to bacterial lawns and needing autoclaves etc.

I've only done a couple of trials but this method seems to work ok, it does require a bit of patience.

I used petri dishes and filter paper but any container and a bit of tissue should work.

The idea is to let the food go off producing bacteria for the amoebal phase to feed off then slowly dry them out so they get persuaded to form…

Continue

Big bang, big disappointment :)

Posted on March 16, 2016 at 17:04 0 Comments

Four weeks of crazy work,  turning the spare room into a culture lab and time lapse studio I made enough culture for two thousand people and I should be there with my friends and colleagues on the stand inspiring the next generation of scientists,  slime fans and eccentrics.  

Unfortunately I'm stuck on a hospital ward with fifty stitches in my neck waiting until I'm allowed home. So it goes I suppose but damn I was looking forward to the Big Bang Fair,  I couldn't even bring a slime…

Continue

Plotting and planning....

Posted on June 2, 2015 at 11:31 0 Comments

Getting involved with several schools in my area running STEM club activities - I'm trying to design a six week course on our dear sticky friend that'll be adaptable to ages 12-17. Looks like it'll be fun ( and a lot of work ). My own cultures have been dormant for a while aside from raising the odd batch but I should start playing again soon. 

Raising slime again..

Posted on May 18, 2015 at 14:17 0 Comments

Blimey it's been a while, we've got the finals lab projects for the students at work on at the moment so I'm not getting time to play. I've got a session with 95 school kids booked in in June so I'm raising as much slime as I can cope with in preparation for that. 

I'm planning on getting them to make Plasticine mazes in petri dishes for their cultures so they've something to take home.

I've also got these intriguing mycena cholorophos spores ( a glowing mushroom ),…

Continue
 
 
 

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