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Gus Xia
  • Hanover, NH
  • United States
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What agar can grow Physarum without oat

Started Oct 13, 2016 0 Replies

Hi,I am new to Physarum Polycephalum. I see in most agar plates, we put oats. I was wondering if any kind of agar plate can grow Live Physarum polycephalum without oats? Especially some prepared ones…Continue

 

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Heather Barnett left a comment for Gus Xia
"It depends what you want to test. Its growth behaviour changes depending on the substrate it is growing on, i.e. it tends to swarm as a mass cell on nutrient agar and branch out in seek mode on non-nutrient agar (I use 2% agar powder or cooking…"
Oct 19, 2016
Gus Xia left a comment for Heather Barnett
"Hi Heather, My name is Gus, a postdoc researcher at Dartmouth College, USA, working on Biocomputing. Our group just stepped in this field and want to discover how different sound/music affect the growth of slime mode. I like you Ted talk and your…"
Oct 17, 2016
Hans-Günther Döbereiner left a comment for Gus Xia
"Dear Gus, please send me an email to hgd@uni-bremen.de Which Group are you in/do you lead ? Best wishes HG"
Oct 14, 2016
Gus Xia posted a discussion

which agar can grow Physarum without oat

Hi,I am new to Physarum Polycephalum. I see in most agar plates, we put oats. I was wondering if any kind of agar plate can grow Live Physarum polycephalum without oats? Especially some prepared ones I can purchase online.I prefer not using oats because I am interested in its shape in a free-growing condition. Putting oats will influence the shape since the slime mold pursues the nutrient. If any preferred nutrient is mixed with the agar evenly, I guess the problem is solved.Thanks a lot!Gus See More
Oct 13, 2016
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Oct 13, 2016
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Oct 13, 2016

Profile Information

Name
Gus
Interest in slime moulds
Shape, acoustic response
Academic Institution (if applicable)
dartmouth college
Website
http://cs.cmu.edu/~gxia

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At 8:22 on October 19, 2016, Heather Barnett said…

It depends what you want to test. Its growth behaviour changes depending on the substrate it is growing on, i.e. it tends to swarm as a mass cell on nutrient agar and branch out in seek mode on non-nutrient agar (I use 2% agar powder or cooking agar). Slime mould will grow on non-nutrient agar but will be more actively looking for food. See https://youtu.be/5tYKYpQzu6E at end when food is taking away its growth becomes more frenetic.

Nutrient rich agar also gets contaminated more quickly.

If you want to try an agar which contains nutrient but isn't visible or lumpy, you could try a mix of yeast extract (Marmite) and dextrose (energy tablets). I have used this as feed, which it loves, but haven't tried mixing it with agar - but I can't see why it wouldn't work.

Try 1 teaspoon of marmite + a third of a dextrose tablet per 100ml of water (recipe comes from Audrey Dussutour via Natalie Andrews). Though I suspect the agar will contaminate quite quickly.

Let me/us (the collective) know how you get on.

I have fed it flour before too, you could try mixing that with agar, it should disperse evenly.

Good luck!

Heather

At 6:15 on October 14, 2016, Hans-Günther Döbereiner said…

Dear Gus,

please send me an email to hgd@uni-bremen.de

Which Group are you in/do you lead ?

Best wishes

HG

 
 
 

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