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İrem
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İrem's Discussions

slime growth is not normal

Started this discussion. Last reply by İrem Apr 4. 6 Replies

I started to produce slime from the filter paper. Currently, slime molds are alive but they do not grow by making connections. As they can be seen in the photo, they spread in a spot-like way and do…Continue

physarum polycephalum not growing

Started this discussion. Last reply by ian Jul 29, 2018. 1 Reply

Hello, i'm an architect and i'm working with physarum polycephalum for my master thesis.I started growing physarum polycephalum 9 months ago. I kept growing 3 months and then i had a break. I wanted…Continue

 

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İrem replied to İrem's discussion slime growth is not normal
"*catabolic"
Apr 4
İrem replied to İrem's discussion slime growth is not normal
"I just wrote wrong. The agar that i use is non- nutrient agar. In the literature, it is reported that slime mold have metabolic depression on agar. I have never used nutrient agar for a period of 1.5 years..."
Apr 3
İrem replied to İrem's discussion slime growth is not normal
"As the contamination rate was very high, I had to transfer it to another petri dish. But in the previous petri dish too, the physarum was not colonized. The agar I use contains nutrients. In fact, I've been working with slime moulds for 1.5…"
Apr 3
ian replied to İrem's discussion slime growth is not normal
"If you've a weak or contaminated sample, it's worth putting a fresh oat right next to one that's colonised. Is your agar just plain agar with no nutrients? - physarum tends to stay put on nutrient agar cheers Ian"
Apr 3
İrem replied to İrem's discussion slime growth is not normal
"Hi Ian,  Because the oats in the previous petri dish were contaminated, I transferred one of the oats with the physarum (left side in the photo) to a new petri dish. In the next process, as seen in the photograph, it is seen that the living…"
Apr 3
ian replied to İrem's discussion slime growth is not normal
"Hi It doesn't look like they've grown at all, the oats don't appear to be colonised. How are you transferring them, to the agar? cheers Ian"
Apr 3
İrem replied to Deimos Denzil's discussion Some tips for my research?
"If you illuminate the area you set as dark as much (too little) as you can observe timelapse, there is no harm to the slimes. I don't think the 3-hour change period is enough for observation"
Mar 28
İrem posted a discussion

slime growth is not normal

I started to produce slime from the filter paper. Currently, slime molds are alive but they do not grow by making connections. As they can be seen in the photo, they spread in a spot-like way and do not leave their own tracks. I had never experienced such a problem while producing live from filter paper before. Is there any idea about the cause of the problem?See More
Mar 28
ian replied to İrem's discussion physarum polycephalum not growing
"Hi  They should keep for longer -  years - but it'll depend on the conditions the sclerotia have been stored in. You could ditch the agar and move to kitchen towel if you want to rule out the agar"
Jul 29, 2018
İrem posted a discussion

physarum polycephalum not growing

Hello, i'm an architect and i'm working with physarum polycephalum for my master thesis.I started growing physarum polycephalum 9 months ago. I kept growing 3 months and then i had a break. I wanted grow them again from sclerotia form last week but the don't grow anymore. Do you have an idea what parameters could be affected physarum? Is this physarum expired just 9 months? or may be agar or oatmeal?Thanks.See More
Jul 24, 2018
İrem is now a member of The Slime Mould Collective
May 4, 2018

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I am an architect and i'm working with physarum polycephalum for my master thesis

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